Proteomic Analysis of Generative and Vegetative Nuclei Reveals Molecular Characteristics of Pollen Cell Differentiation in Lily
文献类型：期刊论文
| 作者 | You, Chen1; Zhang, YuPing; Yang, ShaoYu; Wang, Xu; Yao, Wen; Jin, WeiHuan; Wang, Wei; Hu, XiuLi; Yang, Hao2 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2021 |
| 卷号 | 12 |
| ISSN号 | 1664-462X |
| 关键词 | cell differentiation pollen vegetative cell generative cell nuclear proteins lily proteomic |
| DOI | 10.3389/fpls.2021.641517 |
| 文献子类 | Article |
| 英文摘要 | In plants, the cell fates of a vegetative cell (VC) and generative cell (GC) are determined after the asymmetric division of the haploid microspore. The VC exits the cell cycle and grows a pollen tube, while the GC undergoes further mitosis to produce two sperm cells for double fertilization. However, our understanding of the mechanisms underlying their fate differentiation remains limited. One major advantage of the nuclear proteome analysis is that it is the only method currently able to uncover the systemic differences between VC and GC due to GC being engulfed within the cytoplasm of VC, limiting the use of transcriptome. Here, we obtained pure preparations of the vegetative cell nuclei (VNs) and generative cell nuclei (GNs) from germinating lily pollens. Utilizing these high-purity VNs and GNs, we compared the differential nucleoproteins between them using state-of-the-art quantitative proteomic techniques. We identified 720 different amount proteins (DAPs) and grouped the results in 11 fate differentiation categories. Among them, we identified 29 transcription factors (TFs) and 10 cell fate determinants. Significant differences were found in the molecular activities of vegetative and reproductive nuclei. The TFs in VN mainly participate in pollen tube development. In comparison, the TFs in GN are mainly involved in cell differentiation and male gametogenesis. The identified novel TFs may play an important role in cell fate differentiation. Our data also indicate differences in nuclear pore complexes and epigenetic modifications: more nucleoporins synthesized in VN; more histone variants and chaperones; and structural maintenance of chromosome (SMC) proteins, chromatin remodelers, and DNA methylation-related proteins expressed in GN. The VC has active macromolecular metabolism and mRNA processing, while GC has active nucleic acid metabolism and translation. Moreover, the members of unfolded protein response (UPR) and programmed cell death accumulate in VN, and DNA damage repair is active in GN. Differences in the stress response of DAPs in VN vs. GN were also found. This study provides a further understanding of pollen cell differentiation mechanisms and also a sound basis for future studies of the molecular mechanisms behind cell fate differentiation. |
| 学科主题 | Plant Sciences |
| 出版地 | LAUSANNE |
| WOS关键词 | DNA-BINDING PROTEIN ; MALE GAMETIC CELLS ; TUBE GROWTH ; ARABIDOPSIS-THALIANA ; HISTONE CHAPERONE ; GAMETOPHYTE DEVELOPMENT ; MALE GAMETOGENESIS ; PORE COMPLEX ; TRANSCRIPTION ; GENE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000663825500001 |
| 资助机构 | Youth Program of National Natural Science Foundation of China [31800260] ; Key Laboratory of Plant Molecular Physiology, Chinese Academy of Sciences fund |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26611]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Henan Agr Univ, Coll Life Sci, State Key Lab Wheat & Maize Crop Sci, Zhengzhou, Peoples R China 2.Henan Normal Univ, Coll Life Sci, Xinxiang, Henan, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Chen,Zhang, YuPing,Yang, ShaoYu,et al. Proteomic Analysis of Generative and Vegetative Nuclei Reveals Molecular Characteristics of Pollen Cell Differentiation in Lily[J]. FRONTIERS IN PLANT SCIENCE,2021,12. |
| APA | You, Chen.,Zhang, YuPing.,Yang, ShaoYu.,Wang, Xu.,Yao, Wen.,...&Yang, Hao.(2021).Proteomic Analysis of Generative and Vegetative Nuclei Reveals Molecular Characteristics of Pollen Cell Differentiation in Lily.FRONTIERS IN PLANT SCIENCE,12. |
| MLA | You, Chen,et al."Proteomic Analysis of Generative and Vegetative Nuclei Reveals Molecular Characteristics of Pollen Cell Differentiation in Lily".FRONTIERS IN PLANT SCIENCE 12(2021). |
入库方式： OAI收割
来源：植物研究所
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