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OsGRF6 interacts with SLR1 to regulate OsGA2ox1 expression for coordinating chilling tolerance and growth in rice

文献类型：期刊论文

作者Li, Zhitao3; Wang, Bo3; Zhang, Zeyong; Luo, Wei; Tang, Yongyan; Niu, Yuda; Chong, Kang2; Xu, Yunyuan2
刊名JOURNAL OF PLANT PHYSIOLOGY
出版日期2021
卷号260
关键词Rice Chilling tolerance Gibberellin SLR1 OsGRF6
ISSN号0176-1617
DOI10.1016/j.jplph.2021.153406
文献子类Article
英文摘要Low temperature is one of the abiotic stressors that affect growth and productivity of rice. The plant hormone gibberellin not only regulates growth and development but is also involved in stress defense. Our rice seedling experiments demonstrated that overexpression of SLR1, a gene that encodes the rice DELLA protein, enhanced chilling tolerance. In contrast, overexpression of the active GA synthesis gene OsGA20ox1 reduced chilling tolerance, indicating that weakening GA signaling promoted plant defense against cold stress. CoIP-MS and BiFC assays showed that SLR1 physically interacted with OsGRF6. After cold treatment and recovery, the survival rates of OsGRF6-overexpression lines and an osgrf6 mutant and its complementary lines indicated that OsGRF6 is a negative regulator of chilling tolerance in rice. The yeast one-hybrid, qRT-PCR, and transactivation assays showed that both SLR1 and OsGRF6 can bind to the promoter of the active GA catabolic gene OsGA2ox1, where SLR1 promoted and OsGRF6 suppressed OsGA2ox1 expression. At normal temperature, OsGRF6 was responsible for maintaining active GA levels by inhibiting OsGA2ox1. When rice seedlings were subjected to chilling stress, the repressive effect of OsGRF6 on OsGA2ox1 was released by cold-induced SLR1, which activated OsGA2ox1 expression to decrease the active GA levels, enhancing chilling tolerance. These results suggest that OsGRF6 is an important regulator in the balance between growth and chilling tolerance in rice.
学科主题Plant Sciences
出版地MUNICH
电子版国际标准刊号1618-1328
WOS关键词GIBBERELLIN ; GENE ; TRANSCRIPTION ; PROTEIN ; STRESS ; ORGANOGENESIS ; OSMAPK3 ; TARGETS ; FAMILY ; MODULE
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000642483500002
出版者ELSEVIER GMBH
资助机构National Key Research and Development Program [2016YFD0100901] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA24010301]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26666]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Li, Zhitao,Wang, Bo,Zhang, Zeyong,et al. OsGRF6 interacts with SLR1 to regulate OsGA2ox1 expression for coordinating chilling tolerance and growth in rice[J]. JOURNAL OF PLANT PHYSIOLOGY,2021,260.
APA Li, Zhitao.,Wang, Bo.,Zhang, Zeyong.,Luo, Wei.,Tang, Yongyan.,...&Xu, Yunyuan.(2021).OsGRF6 interacts with SLR1 to regulate OsGA2ox1 expression for coordinating chilling tolerance and growth in rice.JOURNAL OF PLANT PHYSIOLOGY,260.
MLA Li, Zhitao,et al."OsGRF6 interacts with SLR1 to regulate OsGA2ox1 expression for coordinating chilling tolerance and growth in rice".JOURNAL OF PLANT PHYSIOLOGY 260(2021).

入库方式： OAI收割

来源：植物研究所

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