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Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza

文献类型：期刊论文

作者Ma, Ying; Cui, Guanghong; Chen, Tong; Ma, Xiaohui2; Wang, Ruishan; Jin, Baolong; Yang, Jian; Kang, Liping; Tang, Jinfu; Lai, Changjiangsheng
刊名NATURE COMMUNICATIONS
出版日期2021
卷号12期号:1
DOI10.1038/s41467-021-20959-1
文献子类Article
英文摘要Tanshinones are the bioactive nor-diterpenoid constituents of the Chinese medicinal herb Danshen (Salvia miltiorrhiza). These groups of chemicals have the characteristic furan D-ring, which differentiates them from the phenolic abietane-type diterpenoids frequently found in the Lamiaceae family. However, how the 14,16-epoxy is formed has not been elucidated. Here, we report an improved genome assembly of Danshen using a highly homozygous genotype. We identify a cytochrome P450 (CYP71D) tandem gene array through gene expansion analysis. We show that CYP71D373 and CYP71D375 catalyze hydroxylation at carbon-16 (C16) and 14,16-ether (hetero)cyclization to form the D-ring, whereas CYP71D411 catalyzes upstream hydroxylation at C20. In addition, we discover a large biosynthetic gene cluster associated with tanshinone production. Collinearity analysis indicates a more specific origin of tanshinones in Salvia genus. It illustrates the evolutionary origin of abietane-type diterpenoids and those with a furan D-ring in Lamiaceae. Salvia miltiorrhiza is a medicinal plant that can produce the bioactive tanshinones. Here, the authors report the improved genome assembly and reveal the possible roles of three CYP71Ds in catalyzing the reactions leading to the formation of the characteristic furan D-ring of transhinones.
学科主题Multidisciplinary Sciences
出版地BERLIN
电子版国际标准刊号2041-1723
WOS关键词PLANT ; BIOSYNTHESIS ; DANSHEN ; PHARMACOKINETICS ; DIVERSIFICATION ; IDENTIFICATION ; CONSTITUENTS ; CHEMISTRY ; GENES
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000684845600013
出版者NATURE PORTFOLIO
资助机构National Natural Science Foundation of China [81822046, 81573532, 81891010, 81891013, 82003904] ; National Key R&D Program of China [2020YFA0908000, 2018YFA0900600] ; Key project at central government level [2060302] ; Fundamental Research Funds of China Academy of Chinese Medical Sciences [ZZ13-YQ-083, ZZ14-YQ-044] ; NIH [GM131885]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26681]  
专题中科院植物分子生理学重点实验室
作者单位1.Yunnan Univ Tradit Chinese Med, Coll Pharmaceut Sci, Kunming, Yunnan, Peoples R China
2.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Dao Herbs, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
4.Peters, Reuben J.] Iowa State Univ, Roy J Carver Dept Biochem Biophys & Mol Biol, Ames, IA USA
推荐引用方式
GB/T 7714		 Ma, Ying,Cui, Guanghong,Chen, Tong,et al. Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza[J]. NATURE COMMUNICATIONS,2021,12(1).
APA Ma, Ying.,Cui, Guanghong.,Chen, Tong.,Ma, Xiaohui.,Wang, Ruishan.,...&Huang, Luqi.(2021).Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza.NATURE COMMUNICATIONS,12(1).
MLA Ma, Ying,et al."Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza".NATURE COMMUNICATIONS 12.1(2021).

入库方式： OAI收割

来源：植物研究所

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