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Comparative analysis of sRNAs, degradome and transcriptomics in sweet sorghum reveals the regulatory roles of miRNAs in Cd accumulation and tolerance

文献类型：期刊论文

作者Jia, Weitao2; Lin, Kangqi3; Lou, Tengxue3; Feng, Juanjuan; Lv, Sulian; Jiang, Ping; Yi, Ze3; Zhang, Xuan3; Wang, Duoliya3; Guo, Zijing3
刊名PLANTA
出版日期2021
卷号254期号:1
关键词Sweet sorghum Cadmium miRNA Degradome Integrated analysis Co-expression network
ISSN号0032-0935
DOI10.1007/s00425-021-03669-2
文献子类Article
英文摘要Main conclusion Key miRNAs including sbi-miR169p/q, sbi-miR171g/j, sbi-miR172a/c/d, sbi-miR172e, sbi-miR319a/b, sbi-miR396a/b, miR408, sbi-miR5384, sbi-miR5565e and nov_23 were identified to function in the regulation of Cd accumulation and tolerance. As an energy plant, sweet sorghum shows great potential in the phytoremediation of Cd-contaminated soils. However, few studies have focused on the regulatory roles of miRNAs and their targets under Cd stress. In this study, comparative analysis of sRNAs, degradome and transcriptomics was conducted in high-Cd accumulation (H18) and low-Cd accumulation (L69) genotypes of sweet sorghum. A total of 38 conserved and 23 novel miRNAs with differential expressions were identified under Cd stress or between H18 and L69, and 114 target genes of 41 miRNAs were validated. Furthermore, 25 miRNA-mRNA pairs exhibited negatively correlated expression profiles and sbi-miR172e together with its target might participate in the distinct Cd tolerance between H18 and L69 as well as sbi-miR172a/c/d. Additionally, two groups of them: miR169p/q-nov_23 and miR408 were focused through the co-expression analysis, which might be involved in Cd uptake and tolerance by regulating their targets associated with transmembrane transportation, cytoskeleton activity, cell wall construction and ROS (reactive oxygen species) homeostasis. Further experiments exhibited that cell wall components of H18 and L69 were different when exposed to cadmium, which might be regulated by miR169p/q, miR171g/j, miR319a/b, miR396a/b, miR5384 and miR5565e through their targets. Through this study, we aim to reveal the potential miRNAs involved in sweet sorghum in response to Cd stress and provide references for developing high-Cd accumulation or high Cd-resistant germplasm of sweet sorghum that can be used in phytoremediation.
学科主题Plant Sciences
出版地NEW YORK
电子版国际标准刊号1432-2048
WOS关键词GENOME-WIDE IDENTIFICATION ; CADMIUM-RESPONSIVE MICRORNAS ; TARGET GENES ; EXPRESSION ; STRESS ; TOXICITY ; NETWORK ; PLAYERS ; METALS ; LEAVES
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000691583100002
出版者SPRINGER
资助机构National Key Research and Development Program of China [2018YFD0800700] ; Strategic Priority Research Program of Chinese Academy of Sciences [XDA24010306] ; National Natural Science Foundation of China [31971837]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26708]  
专题中科院植物分子生理学重点实验室
作者单位1.Sun Yat Sen Univ, Sch Environm Sci & Engn, Guangdong Prov Key Lab Environm Pollut Control &, Guangzhou 510006, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, 20 Nanxincun, Beijing 100093, Peoples R China
3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm, Chongqing 400714, Peoples R China
4.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Jia, Weitao,Lin, Kangqi,Lou, Tengxue,et al. Comparative analysis of sRNAs, degradome and transcriptomics in sweet sorghum reveals the regulatory roles of miRNAs in Cd accumulation and tolerance[J]. PLANTA,2021,254(1).
APA Jia, Weitao.,Lin, Kangqi.,Lou, Tengxue.,Feng, Juanjuan.,Lv, Sulian.,...&Li, Yinxin.(2021).Comparative analysis of sRNAs, degradome and transcriptomics in sweet sorghum reveals the regulatory roles of miRNAs in Cd accumulation and tolerance.PLANTA,254(1).
MLA Jia, Weitao,et al."Comparative analysis of sRNAs, degradome and transcriptomics in sweet sorghum reveals the regulatory roles of miRNAs in Cd accumulation and tolerance".PLANTA 254.1(2021).

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来源：植物研究所

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