Fine mapping and characterization of two novel quantitative trait loci for early seedling growth in rice
文献类型：期刊论文
| 作者 | Cheng, Peng; Cao, Ling-Jie; Bai, Chen; Ashikari, Motoyuki1; Song, Xian-Jun
|
| 刊名 | PLANTA
 |
| 出版日期 | 2021 |
| 卷号 | 253期号:2 |
| 关键词 | Early seeding growth Leaf size Oryza sativa QTL fine mapping Rice plant |
| ISSN号 | 0032-0935 |
| DOI | 10.1007/s00425-021-03576-6 |
| 文献子类 | Article |
| 英文摘要 | Main conclusionTwo novel QTLs for early seedling growth in rice were fine mapped, with one of which to a 4-kb identical to the known GW6a gene, and another one to a 43-kb region that contains six candidate genes.AbstractLeaves are extremely important for plant photosynthesis: the size and shape of which determine the rate of transpiration, carbon fixation and light interception, and their robust growth at seedling stage endow crops with the ability to compete with weeds. So far, many genes for the traits have been cloned with mutants; however, identification of those quantitative trait loci (QTLs) that control early seedling growth has seldom been reported. In this study, we report the identification of two QTLs, qLBL1 and qLBL2 on the rice chromosome 6 for leaf blade length at early seedling stage. Fine mapping revealed that qLBL1 was placed into a 4-kb, and qLBL2 was delimited to a 43-kb genomic interval. We further found that LBL1 was equivalent to the known grain-size gene GW6a and the qLBL2 region contains 6 candidate genes. Genetic analysis using nearly isogenic lines and transgenic rice plants revealed that both genetic factors were positive regulators. The genetic effects were mainly due to alterations of cell division by cytological observations. RT-qPCR results showed that LBL1 was preferentially expressed in leaf blades, and consistently, histochemical staining of pGW6a::GUS plants showed that GUS signal was strong in the vascular tissues of leaf blade of seedlings. Thus, we fine mapped and characterized two QTLs for early seedling growth and provided useful information to improve crop breeding. |
| 学科主题 | Plant Sciences |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1432-2048 |
| WOS关键词 | FLAG LEAF SIZE ; GENETIC DISSECTION ; CANDIDATE GENES ; YIELD ; ENCODES ; ROOT ; PLASTOCHRON ; MUTATION ; WEIGHT ; ALLELE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000616149400002 |
| 出版者 | SPRINGER |
| 资助机构 | National Key Research and Development Program of China [2016YFD0100402] ; Chinese Academy of Sciences [XDA24010101-2] ; National Natural Science Foundation of China [91735302, 91435113, 31471466] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26762]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China 4.Nagoya Univ, Biosci & Biotechnol Ctr, Nagoya, Aichi 4648601, Japan |
| 推荐引用方式 GB/T 7714 | Cheng, Peng,Cao, Ling-Jie,Bai, Chen,et al. Fine mapping and characterization of two novel quantitative trait loci for early seedling growth in rice[J]. PLANTA,2021,253(2). |
| APA | Cheng, Peng,Cao, Ling-Jie,Bai, Chen,Ashikari, Motoyuki,&Song, Xian-Jun.(2021).Fine mapping and characterization of two novel quantitative trait loci for early seedling growth in rice.PLANTA,253(2). |
| MLA | Cheng, Peng,et al."Fine mapping and characterization of two novel quantitative trait loci for early seedling growth in rice".PLANTA 253.2(2021). |
入库方式： OAI收割
来源：植物研究所
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