TOC1 clock protein phosphorylation controls complex formation with NF-YB/C to repress hypocotyl growth
文献类型：期刊论文
| 作者 | Yan, Jiapei; Li, Shibai1; Kim, Yeon Jeong; Zeng, Qingning; Radziejwoski, Amandine; Wang, Lei6 ; Nomura, Yuko5; Nakagami, Hirofumi4,5; Somers, David E.
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| 刊名 | EMBO JOURNAL
 |
| 出版日期 | 2021 |
| 卷号 | 40期号:24 |
| 关键词 | circadian NUCLEAR FACTOR Y phosphorylation photomorphogenesis TOC1 |
| ISSN号 | 0261-4189 |
| DOI | 10.15252/embj.2021108684 |
| 文献子类 | Article |
| 英文摘要 | Plant photoperiodic growth is coordinated by interactions between circadian clock and light signaling networks. How post-translational modifications of clock proteins affect these interactions to mediate rhythmic growth remains unclear. Here, we identify five phosphorylation sites in the Arabidopsis core clock protein TIMING OF CAB EXPRESSION 1 (TOC1) which when mutated to alanine eliminate detectable phosphorylation. The TOC1 phospho-mutant fails to fully rescue the clock, growth, and flowering phenotypes of the toc1 mutant. Further, the TOC1 phospho-mutant shows advanced phase, a faster degradation rate, reduced interactions with PHYTOCHROME-INTERACTING FACTOR 3 (PIF3) and HISTONE DEACETYLASE 15 (HDA15), and poor binding at pre-dawn hypocotyl growth-related genes (PHGs), leading to a net de-repression of hypocotyl growth. NUCLEAR FACTOR Y subunits B and C (NF-YB/C) stabilize TOC1 at target promoters, and this novel trimeric complex (NF-TOC1) acts as a transcriptional co-repressor with HDA15 to inhibit PIF-mediated hypocotyl elongation. Collectively, we identify a molecular mechanism suggesting how phosphorylation of TOC1 alters its phase, stability, and physical interactions with co-regulators to precisely phase PHG expression to control photoperiodic hypocotyl growth. |
| 学科主题 | Biochemistry & Molecular Biology ; Cell Biology |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1460-2075 |
| WOS关键词 | ARABIDOPSIS CIRCADIAN CLOCK ; POSTTRANSLATIONAL REGULATION ; COINCIDENCE MECHANISM ; MOLECULAR-BASES ; LIGHT ; REGULATOR ; CONSTANS ; PHYTOCHROMES ; ELONGATION ; ZEITLUPE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000713612500001 |
| 出版者 | WILEY |
| 资助机构 | National Institutes of Health [R01GM093285, R35GM136400] ; Next-Generation BioGreen21 Program, the Rural Development Administration, Republic of Korea [PJ01327305] ; World Class University Program of South Korea, NRF, MEST [R31-2008-000-10105-0] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26785]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.[Yan, Jiapei; Li, Shibai; Kim, Yeon Jeong; Zeng, Qingning; Wang, Lei; Somers, David E.] Ohio State Univ, Mol Genet, Columbus, OH 43210 USA 2.Mem Sloan Kettering Canc Ctr, Mol Biol Program, 1275 York Ave, New York, NY 10021 USA 3.Max Planck Inst Plant Breeding Res, Prot Mass Spectrometry, Cologne, Germany 4.RIKEN Ctr Sustainable Resource Sci CSRS, Plant Prote Res Unit, Yokohama, Kanagawa, Japan 5.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 6.Radziejwoski, Amandine; Somers, David E.] POSTECH, Div Integrat Biosci & Biotechnol, Pohang, South Korea |
| 推荐引用方式 GB/T 7714 | Yan, Jiapei,Li, Shibai,Kim, Yeon Jeong,et al. TOC1 clock protein phosphorylation controls complex formation with NF-YB/C to repress hypocotyl growth[J]. EMBO JOURNAL,2021,40(24). |
| APA | Yan, Jiapei.,Li, Shibai.,Kim, Yeon Jeong.,Zeng, Qingning.,Radziejwoski, Amandine.,...&Somers, David E..(2021).TOC1 clock protein phosphorylation controls complex formation with NF-YB/C to repress hypocotyl growth.EMBO JOURNAL,40(24). |
| MLA | Yan, Jiapei,et al."TOC1 clock protein phosphorylation controls complex formation with NF-YB/C to repress hypocotyl growth".EMBO JOURNAL 40.24(2021). |
入库方式： OAI收割
来源：植物研究所
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