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Light-adapted charge-separated state of photosystem II: structural and functional dynamics of the closed reaction center

文献类型：期刊论文

作者Sipka, Gabor; Magyar, Melinda; Mezzetti, Alberto8,9; Akhtar, Parveen1; Zhu, Qingjun; Xiao, Yanan; Han, Guangye; Santabarbara, Stefano5; Shen, Jian-Ren; Lambrev, Petar H.
刊名PLANT CELL
出版日期2021
卷号33期号:4页码:1286-1302
ISSN号1040-4651
DOI10.1093/plcell/koab008
文献子类Article
英文摘要Photosystem II (PSII) uses solar energy to oxidize water and delivers electrons for life on Earth. The photochemical reaction center of PSII is known to possess two stationary states. In the open state (PSIIO), the absorption of a single photon triggers electron-transfer steps, which convert PSII into the charge-separated closed state (PSIIC). Here, by using steady-state and time-resolved spectroscopic techniques on Spinacia oleracea and Thermosynechococcus vulcanus preparations, we show that additional illumination gradually transforms PSIIC into a light-adapted charge-separated state (PSIIL). The PSIIC-to-PSIIL transition, observed at all temperatures between 80 and 308 K, is responsible for a large part of the variable chlorophyll-a fluorescence (F-v) and is associated with subtle, dark-reversible reorganizations in the core complexes, protein conformational changes at noncryogenic temperatures, and marked variations in the rates of photochemical and photophysical reactions. The build-up of PSIIL requires a series of light-induced events generating rapidly recombining primary radical pairs, spaced by sufficient waiting times between these events-pointing to the roles of local electric-field transients and dielectric relaxation processes. We show that the maximum fluorescence level, F-m, is associated with PSIIL rather than with PSIIC, and thus the F-v/F-m parameter cannot be equated with the quantum efficiency of PSII photochemistry. Our findings resolve the controversies and explain the peculiar features of chlorophyll-a fluorescence kinetics, a tool to monitor the functional activity and the structural-functional plasticity of PSII in different wild-types and mutant organisms and under stress conditions.
学科主题Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
出版地CARY
电子版国际标准刊号1532-298X
WOS关键词PHOTOSYNTHETIC REACTION CENTERS ; CHLOROPHYLL FLUORESCENCE ; ELECTRON-TRANSFER ; PICOSECOND FLUORESCENCE ; CONFORMATIONAL-CHANGES ; INFRARED-SPECTROSCOPY ; VARIABLE FLUORESCENCE ; WAVELENGTH DEPENDENCE ; ENERGY-CONVERSION ; PROTEIN
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000671679400042
出版者OXFORD UNIV PRESS INC
资助机构Hungarian Ministry of Innovation and Technology, National Research, Development and Innovation Fund (OTKA) [KH-124985, K-128679, PD-121225, NN-124904, 2018-1.2.1-NKP-2018-00009] ; Hungarian Ministry of Innovation and Technology [TET 2018-2.1.14-TET-CN-2018-00004] ; Czech Science Foundation (GACR) [19-13637S] ; Eotvos Lorand Research Network (ELKH KU) [37/2020] ; COST Actions [CM1306, 40047, CA15126, 41468] ; Grant Fondazione Cariplo (CYAO Project) [2016-0667] ; National Key RAMP ; D Program of China [2017YFA0503700] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB17000000] ; National Natural Science Foundation of China [31470339] ; European Union [GINOP-2.3.6-15-2015-00001] ; European Regional Development Fund
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26331]  
专题中科院光生物学重点实验室
作者单位1.Sorbonne Univ, Lab Reactivit Surface, UMR 7197, Paris, France
2.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama, Japan
3.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama, Japan
4.Natl Res Council Italy, Inst Biophys, Photosynthet Res Unit, Milan, Italy
5.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China
6.ELI HU Nonprofit Ltd, ELI ALPS, Szeged, Hungary
7.Univ Ostrava, Fac Sci, Ostrava, Czech Republic
8.Univ Paris Saclay, CNRS, CEA, Inst Integrat Biol Cell I2BC, F-91191 Gif Sur Yvette, France
9.Inst Plant Biol, Biol Res Ctr, Szeged, Hungary
推荐引用方式
GB/T 7714		 Sipka, Gabor,Magyar, Melinda,Mezzetti, Alberto,et al. Light-adapted charge-separated state of photosystem II: structural and functional dynamics of the closed reaction center[J]. PLANT CELL,2021,33(4):1286-1302.
APA Sipka, Gabor.,Magyar, Melinda.,Mezzetti, Alberto.,Akhtar, Parveen.,Zhu, Qingjun.,...&Garab, Gyozo.(2021).Light-adapted charge-separated state of photosystem II: structural and functional dynamics of the closed reaction center.PLANT CELL,33(4),1286-1302.
MLA Sipka, Gabor,et al."Light-adapted charge-separated state of photosystem II: structural and functional dynamics of the closed reaction center".PLANT CELL 33.4(2021):1286-1302.

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来源：植物研究所

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