Structure of plant photosystem I-light harvesting complex I supercomplex at 2.4 angstrom resolution
文献类型：期刊论文
| 作者 | Wang, Jie2; Yu, Long-Jiang; Wang, Wenda; Yan, Qiujing2; Kuang, Tingyun; Qin, Xiaochun3; Shen, Jian-Ren |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2021 |
| 卷号 | 63期号:7页码:1367-1381 |
| ISSN号 | 1672-9072 |
| 关键词 | crystal structure Lhca LHCI light-harvesting pea photosynthesis photosystem I |
| DOI | 10.1111/jipb.13095 |
| 文献子类 | Article |
| 英文摘要 | Photosystem I (PSI) is one of the two photosystems in photosynthesis, and performs a series of electron transfer reactions leading to the reduction of ferredoxin. In higher plants, PSI is surrounded by four light-harvesting complex I (LHCI) subunits, which harvest and transfer energy efficiently to the PSI core. The crystal structure of PSI-LHCI supercomplex has been analyzed up to 2.6 angstrom resolution, providing much information on the arrangement of proteins and cofactors in this complicated supercomplex. Here we have optimized crystallization conditions, and analyzed the crystal structure of PSI-LHCI at 2.4 angstrom resolution. Our structure showed some shift of the LHCI, especially the Lhca4 subunit, away from the PSI core, suggesting the indirect connection and inefficiency of energy transfer from this Lhca subunit to the PSI core. We identified five new lipids in the structure, most of them are located in the gap region between the Lhca subunits and the PSI core. These lipid molecules may play important roles in binding of the Lhca subunits to the core, as well as in the assembly of the supercomplex. The present results thus provide novel information for the elucidation of the mechanisms for the light-energy harvesting, transfer and assembly of this supercomplex. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 电子版国际标准刊号 | 1744-7909 |
| 出版地 | HOBOKEN |
| WOS关键词 | ENERGY-TRANSFER PATHWAYS ; ANTENNA ARRANGEMENT ; PSI-LHCI ; IDENTIFICATION ; INTERMEDIATE ; LIPIDS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000656625200001 |
| 资助机构 | National Key Research and Development Program of China [2017YFA0503700] ; Chinese Academy of Sciences Key Research Program of Frontier Sciences [QYZDY-SSW-SMC003] ; Strategic Priority Research Program of CAS [XDB17000000] ; National Basic Research Program of China [2015CB150100] ; National Natural Science Foundation of China [31622007, 31670237, 32070267] ; Youth Innovation Promotion Association of CAS [2020081] ; Jinan Science and Technology Project [2020GXRC058] ; Natural Science Foundation of Shandong Province China [ZR2019ZD48] ; Taishan Scholars Project |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26338]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Jinan Univ, Sch Biol Sci & Technol, Jinan 250022, Peoples R China 5.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama 7008530, Japan |
| 推荐引用方式 GB/T 7714 | Wang, Jie,Yu, Long-Jiang,Wang, Wenda,et al. Structure of plant photosystem I-light harvesting complex I supercomplex at 2.4 angstrom resolution[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2021,63(7):1367-1381. |
| APA | Wang, Jie.,Yu, Long-Jiang.,Wang, Wenda.,Yan, Qiujing.,Kuang, Tingyun.,...&Shen, Jian-Ren.(2021).Structure of plant photosystem I-light harvesting complex I supercomplex at 2.4 angstrom resolution.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,63(7),1367-1381. |
| MLA | Wang, Jie,et al."Structure of plant photosystem I-light harvesting complex I supercomplex at 2.4 angstrom resolution".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 63.7(2021):1367-1381. |
入库方式： OAI收割
来源：植物研究所
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