Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4
文献类型：期刊论文
| 作者 | Hu, Jiu-Hui; Chang, Jing-Wen; Xu, Tao; Wang, Jia1; Wang, Xiao4; Lin, Rongcheng1; Duanmu, Deqiang3; Liu, Lin4 |
| 刊名 | PROTEIN SCIENCE
 |
| 出版日期 | 2021 |
| 卷号 | 30期号:10页码:2083-2091 |
| ISSN号 | 0961-8368 |
| 关键词 | biliverdin phycocyanobilin retrograde signaling tetrapyrrole |
| DOI | 10.1002/pro.4164 |
| 文献子类 | Article |
| 英文摘要 | The chlorophyll biosynthesis regulator GENOMES UNCOUPLED 4 (GUN4) is conserved in nearly all oxygenic photosynthetic organisms. Recently, GUN4 has been found to be able to bind the linear tetrapyrroles (bilins) and stimulate the magnesium chelatase activity in the unicellular green alga Chlamydomonas reinhardtii. Here, we characterize GUN4 proteins from Arabidopsis thaliana and the cyanobacterium Synechocystis sp. PCC 6803 for their ability to bind bilins, and present the crystal structures of Synechocystis GUN4 in biliverdin-bound, phycocyanobilin-bound, and phytochromobilin-bound forms at the resolutions of 1.05, 1.10, and 1.70 angstrom, respectively. These linear molecules adopt a cyclic-helical conformation, and bind more tightly than planar porphyrins to the tetrapyrrole-binding pocket of GUN4. Based on structural comparison, we propose a working model of GUN4 in regulation of tetrapyrrole biosynthetic pathway, and address the role of the bilin-bound GUN4 in retrograde signaling. |
| 学科主题 | Biochemistry & Molecular Biology |
| 电子版国际标准刊号 | 1469-896X |
| 出版地 | HOBOKEN |
| WOS关键词 | GENOMES UNCOUPLED 4 ; MG-CHELATASE ; STRUCTURE REFINEMENT ; GENE-EXPRESSION ; SUBUNIT ; PROTEIN ; PROMOTE ; MUTANT ; MODEL |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000686926700001 |
| 资助机构 | Anhui Provincial Wan Jiang Scholars Program ; Ministry of Science and Technology of the People's Republic of China [2017YFA0503703] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26390]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Anhui Univ, Sch Life Sci, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China 2.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China 3.Anhui Univ, Anhui Key Lab Modern Biomfg, Hefei, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Jiu-Hui,Chang, Jing-Wen,Xu, Tao,et al. Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4[J]. PROTEIN SCIENCE,2021,30(10):2083-2091. |
| APA | Hu, Jiu-Hui.,Chang, Jing-Wen.,Xu, Tao.,Wang, Jia.,Wang, Xiao.,...&Liu, Lin.(2021).Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4.PROTEIN SCIENCE,30(10),2083-2091. |
| MLA | Hu, Jiu-Hui,et al."Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4".PROTEIN SCIENCE 30.10(2021):2083-2091. |
入库方式： OAI收割
来源：植物研究所
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