Construction and application of easy-to-detect cyanobacteria with vp28 gene
文献类型：期刊论文
| 作者 | Zhuang, Min-Min; Peng, Wen; Xu, Yang; Jia, Xiao-Hui; Shi, Ding-Ji; He, Pei-Min; Jia, Rui3 |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2021 |
| 卷号 | 33期号:4页码:2341-2348 |
| 关键词 | vp28 gene egfp gene Synechococcus elongatus PCC 7942 WSSV |
| ISSN号 | 0921-8971 |
| DOI | 10.1007/s10811-021-02414-2 |
| 文献子类 | Article |
| 英文摘要 | White Spot Syndrome Virus (WSSV) is the most harmful and pathogenic infectious agent in shrimp aquaculture. Vp28, a protein used to protect hosts against this virus, has successfully been expressed in nine hosts since 2004; however, to date, it has not been used commercially. We constructed transgenic Synechococcus elongatus PCC 7942 expressing vp28 and enhanced green fluorescent protein (egfp) genes simultaneously to enable the easy detection of vp28 expression. GFP has been expressed in cyanobacteria previously and was used in this study to construct transgenic cyanobacteria that can easily be detected. The optimal growth conditions for the transgenic cyanobacteria were determined using a liquid-phase oxygen electrode. At the optimum light intensity, the net photosynthetic rate of the transgenic cyanobacteria increased by 12.6% compared with that of the wild-type. Furthermore, Synechococcus sp. PCC 7942 harboring vp28 and vp28-egfp were fed to shrimp in order to protect them against WSSV infection. The cumulative survival rate of the groups fed with transgenic cyanobacteria was 75%. |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 出版地 | DORDRECHT |
| 电子版国际标准刊号 | 1573-5176 |
| WOS关键词 | SPOT SYNDROME VIRUS ; VECTORS ; BIOLOGY ; DESIGN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000655833100001 |
| 出版者 | SPRINGER |
| 资助机构 | National Key R&D Program of China [2019YFC0312604] ; shanghai Agriculture Science and Technology Innovation Project (2017) [1-13] ; Shanghai Science and Technology Commission Innovation Project [17391902200] ; National High Technology Research and Development Program of China (863 Program) [2014AA093506] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26402]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.[Peng, Wen 2.Xu, Yang 3.He, Pei-Min 4.Shanghai Ocean Univ, Coll Marine Ecol & Environm, Shanghai 201306, Peoples R China 5.Beijing Normal Univ, Beijing 100875, Peoples R China 6.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 7.East China Normal Univ, Shanghai 200241, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhuang, Min-Min,Peng, Wen,Xu, Yang,et al. Construction and application of easy-to-detect cyanobacteria with vp28 gene[J]. JOURNAL OF APPLIED PHYCOLOGY,2021,33(4):2341-2348. |
| APA | Zhuang, Min-Min.,Peng, Wen.,Xu, Yang.,Jia, Xiao-Hui.,Shi, Ding-Ji.,...&Jia, Rui.(2021).Construction and application of easy-to-detect cyanobacteria with vp28 gene.JOURNAL OF APPLIED PHYCOLOGY,33(4),2341-2348. |
| MLA | Zhuang, Min-Min,et al."Construction and application of easy-to-detect cyanobacteria with vp28 gene".JOURNAL OF APPLIED PHYCOLOGY 33.4(2021):2341-2348. |
入库方式： OAI收割
来源：植物研究所
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