Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum
文献类型：期刊论文
| 作者 | Tani, Kazutoshi3; Kanno, Ryo; Ji, Xuan-Cheng; Hall, Malgorzata; Yu, Long-Jiang ; Kimura, Yukihiro5; Madigan, Michael T.; Mizoguchi, Akira3; Humbel, Bruno M.; Wang-Otomo, Zheng-Yu
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| 刊名 | BIOCHEMISTRY
 |
| 出版日期 | 2021 |
| 卷号 | 60期号:32页码:2483-2491 |
| ISSN号 | 0006-2960 |
| DOI | 10.1021/acs.biochem.1c00360 |
| 文献子类 | Article |
| 英文摘要 | Rhodospirillum (Rsp.) rubrum is one of the most widely used model organisms in bacterial photosynthesis. This purple phototroph is characterized by the presence of both rhodoquinone (RQ) and ubiquinone as electron carriers and bacteriochlorophyll (BChl) a esterified at the propionic acid side chain by geranylgeraniol (BChl a(G)) instead of phytol. Despite intensive efforts, the structure of the light-harvesting-reaction center (LH1-RC) core complex from Rsp. rubrum remains at low resolutions. Using cryo-EM, here we present a robust new view of the Rsp. rubrum LH1-RC at 2.76 angstrom resolution. The LH1 complex forms a closed, slightly elliptical ring structure with 16 alpha beta-polypeptides surrounding the RC. Our biochemical analysis detected RQ molecules in the purified LH1-RC, and the cryo-EM density map specifically positions RQ at the Q(A) site in the RC. The geranylgeraniol side chains of BChl a(G) coordinated by LH1 beta-polypeptides exhibit a highly homologous tail-up conformation that allows for interactions with the bacteriochlorin rings of nearby LH1 alpha-associated BChls a(G). The structure also revealed key protein-protein interactions in both Nand C-terminal regions of the LH1 alpha beta-polypeptides, mainly within a face-to-face structural subunit. Our high-resolution Rsp. rubrum LH1-RC structure provides new insight for evaluating past experimental and computational results obtained with this old organism over many decades and lays the foundation for more detailed exploration of light-energy conversion, quinone transport, and structure-function relationships in this pigment-protein complex. |
| 学科主题 | Biochemistry & Molecular Biology |
| 出版地 | WASHINGTON |
| WOS关键词 | LIGHT-HARVESTING COMPLEX ; CHEMICALLY SYNTHESIZED POLYPEPTIDES ; IN-VITRO RECONSTITUTION ; SUBUNIT FORM ; B820 SUBUNIT ; BACTERIOCHLOROPHYLL BINDING ; RHODOBACTER-SPHAEROIDES ; MINIMAL REQUIREMENTS ; ALPHA-POLYPEPTIDE ; BETA-POLYPEPTIDE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000687059000005 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED [JP21am0101118, 1758, JP21am0101116, 1878, 17am0101116, 18am0101116, 19am0101116, 20am0101116] ; Okinawa Institute of Science and Technology ; National Key R&D Program of China [2019YFA0904600] ; JSPS KAKENHI grant [JP16H04174, JP18H05153, JP20H05086, JP20H02856] ; Takeda Science Foundation ; Kurata Memorial Hitachi Science and Technology Foundation, Japan ; Japanese Cabinet Office |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26407]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Ibaraki Univ, Fac Sci, Mito, Ibaraki 3108512, Japan 2.Mie Univ, Grad Sch Med, Tsu, Mie 5148507, Japan 3.Madigan, Michael T.] Southern Illinois Univ, Sch Biol Sci, Carbondale, IL 62901 USA 4.Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Kobe, Hyogo 6578501, Japan 5.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photo Biol, Beijing 100093, Peoples R China 6.[Kanno, Ryo; Hall, Malgorzata; Humbel, Bruno M.] Okinawa Inst Sci & Technol Grad Univ OIST, Res Support Div, Imaging Sect, Onna, Okinawa 9040495, Japan |
| 推荐引用方式 GB/T 7714 | Tani, Kazutoshi,Kanno, Ryo,Ji, Xuan-Cheng,et al. Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum[J]. BIOCHEMISTRY,2021,60(32):2483-2491. |
| APA | Tani, Kazutoshi.,Kanno, Ryo.,Ji, Xuan-Cheng.,Hall, Malgorzata.,Yu, Long-Jiang.,...&Wang-Otomo, Zheng-Yu.(2021).Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum.BIOCHEMISTRY,60(32),2483-2491. |
| MLA | Tani, Kazutoshi,et al."Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum".BIOCHEMISTRY 60.32(2021):2483-2491. |
入库方式： OAI收割
来源：植物研究所
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