Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes
文献类型：期刊论文
| 作者 | Kimura, Y.; Yamashita, T.; Seto, R.; Imanishi, M.; Honda, M.; Nakagawa, S.; Saga, Y.; Takenaka, S.; Yu, L. -J.; Madigan, M. T. |
| 刊名 | PHOTOSYNTHESIS RESEARCH
 |
| 出版日期 | 2021 |
| 卷号 | 148期号:1-2页码:77-86 |
| ISSN号 | 0166-8595 |
| 关键词 | Light-harvesting Reaction center Hydrogen bond π – π interaction Redshift ultra-redshift |
| DOI | 10.1007/s11120-021-00831-5 |
| 文献子类 | Article |
| 英文摘要 | The core light-harvesting complexes (LH1) in bacteriochlorophyll (BChl) b-containing purple phototrophic bacteria are characterized by a near-infrared absorption maximum around 1010 nm. The determinative cause for this ultra-redshift remains unclear. Here, we present results of circular dichroism (CD) and resonance Raman measurements on the purified LH1 complexes in a reaction center-associated form from a mesophilic and a thermophilic Blastochloris species. Both the LH1 complexes displayed purely positive CD signals for their Q(y) transitions, in contrast to those of BChl a-containing LH1 complexes. This may reflect differences in the conjugation system of the bacteriochlorin between BChl b and BChl a and/or the differences in the pigment organization between the BChl b- and BChl a-containing LH1 complexes. Resonance Raman spectroscopy revealed remarkably large redshifts of the Raman bands for the BChl b C3-acetyl group, indicating unusually strong hydrogen bonds formed with LH1 polypeptides, results that were verified by a published structure. A linear correlation was found between the redshift of the Raman band for the BChl C3-acetyl group and the change in LH1-Q(y) transition for all native BChl a- and BChl b-containing LH1 complexes examined. The strong hydrogen bonding and pi-pi interactions between BChl b and nearby aromatic residues in the LH1 polypeptides, along with the CD results, provide crucial insights into the spectral and structural origins for the ultra-redshift of the long-wavelength absorption maximum of BChl b-containing phototrophs. |
| 学科主题 | Plant Sciences |
| 电子版国际标准刊号 | 1573-5079 |
| 出版地 | DORDRECHT |
| WOS关键词 | CORE COMPLEX |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000638054400001 |
| 资助机构 | JSPS KAKENHI [JP16H04174, JP18H05153, JP20H05086, JP20H02856] ; Takeda Science Foundation ; Kurata Memorial Hitachi Science and Technology Foundation, Japan ; National Key R&D Program of China [2019YFA0904600] ; [19K06563] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26504]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Wang-Otomo, Z. -Y.] Ibaraki Univ, Fac Sci, Mito, Ibaraki 3108512, Japan 2.[Kimura, Y. 3.Seto, R. 4.Imanishi, M. 5.Takenaka, S.] Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Nada Ku, Kobe, Hyogo 6578501, Japan 6.[Yamashita, T. 7.Honda, M. 8.[Nakagawa, S. 9.Saga, Y.] Kindai Univ, Dept Chem, Higashiosaka, Osaka 5778502, Japan 10.[Yu, L. -J.] Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kimura, Y.,Yamashita, T.,Seto, R.,et al. Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes[J]. PHOTOSYNTHESIS RESEARCH,2021,148(1-2):77-86. |
| APA | Kimura, Y..,Yamashita, T..,Seto, R..,Imanishi, M..,Honda, M..,...&Wang-Otomo, Z. -Y..(2021).Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes.PHOTOSYNTHESIS RESEARCH,148(1-2),77-86. |
| MLA | Kimura, Y.,et al."Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes".PHOTOSYNTHESIS RESEARCH 148.1-2(2021):77-86. |
入库方式： OAI收割
来源：植物研究所
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