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Plastid Deficient 1 Is Essential for the Accumulation of Plastid-Encoded RNA Polymerase Core Subunit beta and Chloroplast Development in Arabidopsis

文献类型：期刊论文

作者Yang, Zhipan; Liu, Mingxin2; Ding, Shunhua; Zhang, Yi; Yang, Huixia; Wen, Xiaogang; Chi, Wei; Lu, Congming1; Lu, Qingtao2
刊名INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
出版日期2021
卷号22期号:24
关键词Arabidopsis PD1 PEP chloroplast development chloroplast gene expression
DOI10.3390/ijms222413648
文献子类Article
英文摘要Plastid-encoded RNA polymerase (PEP)-dependent transcription is an essential process for chloroplast development and plant growth. It is a complex event that is regulated by numerous nuclear-encoded proteins. In order to elucidate the complex regulation mechanism of PEP activity, identification and characterization of PEP activity regulation factors are needed. Here, we characterize Plastid Deficient 1 (PD1) as a novel regulator for PEP-dependent gene expression and chloroplast development in Arabidopsis. The PD1 gene encodes a protein that is conserved in photoautotrophic organisms. The Arabidopsis pd1 mutant showed albino and seedling-lethal phenotypes. The plastid development in the pd1 mutant was arrested. The PD1 protein localized in the chloroplasts, and it colocalized with nucleoid protein TRXz. RT-quantitative real-time PCR, northern blot, and run-on analyses indicated that the PEP-dependent transcription in the pd1 mutant was dramatically impaired, whereas the nuclear-encoded RNA polymerase-dependent transcription was up-regulated. The yeast two-hybrid assays and coimmunoprecipitation experiments showed that the PD1 protein interacts with PEP core subunit beta (PEP-beta), which has been verified to be essential for chloroplast development. The immunoblot analysis indicated that the accumulation of PEP-beta was barely detected in the pd1 mutant, whereas the accumulation of the other essential components of the PEP complex, such as core subunits alpha and beta ', were not affected in the pd1 mutant. These observations suggested that the PD1 protein is essential for the accumulation of PEP-beta and chloroplast development in Arabidopsis, potentially by direct interaction with PEP-beta.
学科主题Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
出版地BASEL
电子版国际标准刊号1422-0067
WOS关键词PENTATRICOPEPTIDE REPEAT PROTEINS ; ACTIVE CHROMOSOME COMPLEX ; GENE-EXPRESSION ; NUCLEOID PROTEIN ; REDOX REGULATION ; TRANSCRIPTION ; INTERACTS ; BIOGENESIS ; COMPONENT ; REDUCTASE
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000739060500001
出版者MDPI
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26546]  
专题中科院光生物学重点实验室
作者单位1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
3.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Zhipan,Liu, Mingxin,Ding, Shunhua,et al. Plastid Deficient 1 Is Essential for the Accumulation of Plastid-Encoded RNA Polymerase Core Subunit beta and Chloroplast Development in Arabidopsis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(24).
APA Yang, Zhipan.,Liu, Mingxin.,Ding, Shunhua.,Zhang, Yi.,Yang, Huixia.,...&Lu, Qingtao.(2021).Plastid Deficient 1 Is Essential for the Accumulation of Plastid-Encoded RNA Polymerase Core Subunit beta and Chloroplast Development in Arabidopsis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(24).
MLA Yang, Zhipan,et al."Plastid Deficient 1 Is Essential for the Accumulation of Plastid-Encoded RNA Polymerase Core Subunit beta and Chloroplast Development in Arabidopsis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.24(2021).

入库方式： OAI收割

来源：植物研究所

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