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Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063

文献类型：期刊论文

作者Xiaol, Yanan6; Huang, Guoqiang; You, Xin; Zhu, Qingjun6; Wang, Wenda; Kuang, Tingyun; Han, Guangye; Sui, Sen-Fang; Shen, Jian-Ren
刊名NATURE PLANTS
出版日期2021
卷号7期号:8页码:1132-1142
ISSN号2055-026X
DOI10.1038/s41477-021-00961-7
文献子类Article
英文摘要Cryo-electron microscopy structures of two PSII intermediates from psbV-deletion and wild-type strains of cyanobacteria show substantial structural changes. These provide a structural basis for understanding the assembly process of native PSII. Photosystem II (PSII) is a multisubunit pigment-protein complex and catalyses light-induced water oxidation, leading to the conversion of light energy into chemical energy and the release of dioxygen. We analysed the structures of two Psb28-bound PSII intermediates, Psb28-RC47 and Psb28-PSII, purified from a psbV-deletion strain of the thermophilic cyanobacterium Thermosynechococcus vulcanus, using cryo-electron microscopy. Both Psb28-RC47 and Psb28-PSII bind one Psb28, one Tsl0063 and an unknown subunit. Psb28 is located at the cytoplasmic surface of PSII and interacts with D1, D2 and CP47, whereas Tsl0063 is a transmembrane subunit and binds at the side of CP47/PsbH. Substantial structural perturbations are observed at the acceptor side, which result in conformational changes of the quinone (Q(B)) and non-haem iron binding sites and thus may protect PSII from photodamage during assembly. These results provide a solid structural basis for understanding the assembly process of native PSII.
学科主题Plant Sciences
出版地BERLIN
电子版国际标准刊号2055-0278
WOS关键词CAB-LIKE PROTEINS ; SYNECHOCYSTIS-SP PCC-6803 ; CRYO-EM STRUCTURE ; SP PCC 6803 ; LIGHT-INDUCIBLE POLYPEPTIDES ; CRYSTAL-STRUCTURE ; WATER-OXIDATION ; CHLOROPHYLL ; COMPLEX ; OXYGEN
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000669838400005
出版者NATURE PORTFOLIO
资助机构National Key R&D Program of China [2017YFA0503700, 2016YFA0501101, 2017YFA0504600, 2020YFA0907600, 2019YFA0906300] ; National Natural Science Foundation of China [31470339] ; Strategic Priority Research Program of CAS [XDA27050402, XDB17000000] ; CAS Key Research programme for Frontier Science [QYZDY-SSW-SMC003] ; Youth Innovation Promotion Association of CAS [2020081] ; CAS Interdisciplinary Innovation Team [JCTD-2020-06]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26556]  
专题中科院光生物学重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Tsinghua Univ, Frontier Res Ctr Biol Struct, Sch Life Sci, Beijing, Peoples R China
3.Southern Univ Sci & Technol, Dept Biol, Shenzhen, Peoples R China
4.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama, Japan
5.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama, Japan
6.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China
7.Tsinghua Univ, Sch Life Sci, State Key Lab Membrane Biol, Beijing Adv Innovat Ctr Struct Biol, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Xiaol, Yanan,Huang, Guoqiang,You, Xin,et al. Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063[J]. NATURE PLANTS,2021,7(8):1132-1142.
APA Xiaol, Yanan.,Huang, Guoqiang.,You, Xin.,Zhu, Qingjun.,Wang, Wenda.,...&Shen, Jian-Ren.(2021).Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063.NATURE PLANTS,7(8),1132-1142.
MLA Xiaol, Yanan,et al."Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063".NATURE PLANTS 7.8(2021):1132-1142.

入库方式： OAI收割

来源：植物研究所

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