A previously unrecognized membrane protein in the Rhodobacter sphaeroides LH1-RC photocomplex
文献类型：期刊论文
| 作者 | Tani, Kazutoshi; Nagashima, Kenji V. P.; Kanno, Ryo; Kawamura, Saki; Kikuchi, Riku; Hall, Malgorzata; Yu, Long-Jiang ; Kimura, Yukihiro7; Madigan, Michael T.; Mizoguchi, Akira
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| 刊名 | NATURE COMMUNICATIONS
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| 出版日期 | 2021 |
| 卷号 | 12期号:1 |
| DOI | 10.1038/s41467-021-26561-9 |
| 文献子类 | Article |
| 英文摘要 | Rhodobacter (Rba.) sphaeroides is the most widely used model organism in bacterial photosynthesis. The light-harvesting-reaction center (LH1-RC) core complex of this purple phototroph is characterized by the co-existence of monomeric and dimeric forms, the presence of the protein PufX, and approximately two carotenoids per LH1 alpha beta-polypeptides. Despite many efforts, structures of the Rba. sphaeroides LH1-RC have not been obtained at high resolutions. Here we report a cryo-EM structure of the monomeric LH1-RC from Rba. sphaeroides strain IL106 at 2.9 angstrom resolution. The LH1 complex forms a C-shaped structure composed of 14 alpha beta-polypeptides around the RC with a large ring opening. From the cryo-EM density map, a previously unrecognized integral membrane protein, referred to as protein-U, was identified. Protein-U has a U-shaped conformation near the LH1-ring opening and was annotated as a hypothetical protein in the Rba. sphaeroides genome. Deletion of protein-U resulted in a mutant strain that expressed a much-reduced amount of the dimeric LH1-RC, indicating an important role for protein-U in dimerization of the LH1-RC complex. PufX was located opposite protein-U on the LH1-ring opening, and both its position and conformation differed from that of previous reports of dimeric LH1-RC structures obtained at low-resolution. Twenty-six molecules of the carotenoid spheroidene arranged in two distinct configurations were resolved in the Rba. sphaeroides LH1 and were positioned within the complex to block its channels. Our findings offer an exciting new view of the core photocomplex of Rba. sphaeroides and the connections between structure and function in bacterial photocomplexes in general. Rhodobacter (Rba.) sphaeroides is a model organism for studying bacterial photosynthesis. Here, the authors present the 2.9 angstrom cryo-EM structure of the monomeric light-harvesting-reaction center core complex from Rba. sphaeroides strain IL106, which revealed the position and conformation of PufX and the presence of an additional component protein-U, an integral membrane protein. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | BERLIN |
| 电子版国际标准刊号 | 2041-1723 |
| WOS关键词 | PHOTOSYNTHETIC CORE COMPLEX ; PUFX POLYPEPTIDE ; RHODOPSEUDOMONAS-SPHAEROIDES ; NATIVE ARCHITECTURE ; RC-LH1-PUFX COMPLEX ; DIMERIZATION ; ANTENNA ; HELIX ; COMPLEMENTATION ; CONFORMATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000714189200023 |
| 出版者 | NATURE PORTFOLIO |
| 资助机构 | AMED [JP20am0101118, 1758, JP20am0101116, 1878, 17am0101116j0001, 18am0101116j0002, 19am0101116j0003] ; Okinawa Institute of Science and Technology ; Japanese Cabinet Office ; National Key R&D Program of China [2019YFA0904600] ; JSPS KAKENHI [JP16H04174, JP18H05153, JP20H05086, JP20H02856] ; Takeda Science Foundation ; Kurata Memorial Hitachi Science and Technology Foundation, Japan |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26587]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Ibaraki Univ, Fac Sci, Mito, Ibaraki 3108512, Japan 2.Mie Univ, Grad Sch Med, Tsu, Mie 5148507, Japan 3.Nagashima, Kenji V. P.] Kanagawa Univ, Res Inst Integrated Sci, 2946 Tsuchiya, Hiratsuka, Kanagawa 2591293, Japan 4.Kanno, Ryo; Hall, Malgorzata; Humbel, Bruno M.] Okinawa Inst Sci & Technol Grad Univ OIST, Res Support Div, Imaging Sect, 1919-1 Tancha, Onna Son, Okinawa 9040495, Japan 5.Madigan, Michael T.] Southern Illinois Univ, Sch Biol Sci, Dept Microbiol, Carbondale, IL 62901 USA 6.Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Nada Ku, Kobe, Hyogo 6578501, Japan 7.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tani, Kazutoshi,Nagashima, Kenji V. P.,Kanno, Ryo,et al. A previously unrecognized membrane protein in the Rhodobacter sphaeroides LH1-RC photocomplex[J]. NATURE COMMUNICATIONS,2021,12(1). |
| APA | Tani, Kazutoshi.,Nagashima, Kenji V. P..,Kanno, Ryo.,Kawamura, Saki.,Kikuchi, Riku.,...&Wang-Otomo, Zheng-Yu.(2021).A previously unrecognized membrane protein in the Rhodobacter sphaeroides LH1-RC photocomplex.NATURE COMMUNICATIONS,12(1). |
| MLA | Tani, Kazutoshi,et al."A previously unrecognized membrane protein in the Rhodobacter sphaeroides LH1-RC photocomplex".NATURE COMMUNICATIONS 12.1(2021). |
入库方式： OAI收割
来源：植物研究所
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