Crystal structure of a photosynthetic LH1-RC in complex with its electron donor HiPIP
文献类型：期刊论文
| 作者 | Kawakami, Tomoaki; Yu, Long-Jiang ; Liang, Tai; Okazaki, Koudai; Madigan, Michael T.; Kimura, Yukihiro2; Wang-Otomo, Zheng-Yu
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| 刊名 | NATURE COMMUNICATIONS
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| 出版日期 | 2021 |
| 卷号 | 12期号:1 |
| DOI | 10.1038/s41467-021-21397-9 |
| 文献子类 | Article |
| 英文摘要 | Photosynthetic electron transfers occur through multiple components ranging from small soluble proteins to large integral membrane protein complexes. Co-crystallization of a bacterial photosynthetic electron transfer complex that employs weak hydrophobic interactions was achieved by using high-molar-ratio mixtures of a soluble donor protein (high-potential iron-sulfur protein, HiPIP) with a membrane-embedded acceptor protein (reaction center, RC) at acidic pH. The structure of the co-complex offers a snapshot of a transient bioenergetic event and revealed a molecular basis for thermodynamically unfavorable interprotein electron tunneling. HiPIP binds to the surface of the tetraheme cytochrome subunit in the light-harvesting (LH1) complex-associated RC in close proximity to the low-potential heme-1 group. The binding interface between the two proteins is primarily formed by uncharged residues and is characterized by hydrophobic features. This co-crystal structure provides a model for the detailed study of long-range trans-protein electron tunneling pathways in biological systems. The high potential iron-sulfur (HiPIP) proteins are direct electron donors to the light-harvesting-reaction center complexes (LH1-RC) in photosynthetic beta- and gamma -Proteobacteria. Here, the authors present the 2.9 angstrom crystal structure of the HiPIP-bound LH1-RC complex from the thermophilic purple sulfur bacterium Thermochromatium tepidum and discuss mechanistic implications for the electron transfer pathway. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | BERLIN |
| 电子版国际标准刊号 | 2041-1723 |
| WOS关键词 | TETRAHEME CYTOCHROME SUBUNIT ; IRON-SULFUR PROTEIN ; INTERACTION SITE ; BACTERIUM ; BINDING ; C(2) ; THERMOSTABILITY ; THERMODYNAMICS ; COMPENSATION ; FERREDOXIN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000621232800023 |
| 出版者 | NATURE PORTFOLIO |
| 资助机构 | JSPS KAKENHI [JP16H04174, JP18H05153, JP20H05086, JP20H02856] ; Takeda Science Foundation ; Kurata Memorial Hitachi Science and Technology Foundation, Japan ; grant of KINOU-KYOKA from Institute of Quantum Beam Science of Ibaraki University ; National Key R&D Program of China [2019YFA0904600] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26704]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing, Peoples R China 2.Madigan, Michael T.] Southern Illinois Univ, Dept Microbiol, Carbondale, IL USA 3.Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Kobe, Hyogo, Japan 4.Ibaraki Univ, Fac Sci, Mito, Ibaraki, Japan |
| 推荐引用方式 GB/T 7714 | Kawakami, Tomoaki,Yu, Long-Jiang,Liang, Tai,et al. Crystal structure of a photosynthetic LH1-RC in complex with its electron donor HiPIP[J]. NATURE COMMUNICATIONS,2021,12(1). |
| APA | Kawakami, Tomoaki.,Yu, Long-Jiang.,Liang, Tai.,Okazaki, Koudai.,Madigan, Michael T..,...&Wang-Otomo, Zheng-Yu.(2021).Crystal structure of a photosynthetic LH1-RC in complex with its electron donor HiPIP.NATURE COMMUNICATIONS,12(1). |
| MLA | Kawakami, Tomoaki,et al."Crystal structure of a photosynthetic LH1-RC in complex with its electron donor HiPIP".NATURE COMMUNICATIONS 12.1(2021). |
入库方式： OAI收割
来源：植物研究所
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