Electrostatic charge controls the lowest LH1 Q(y) transition energy in the triply extremophilic purple phototrophic bacterium, Halorhodospira halochloris
文献类型：期刊论文
| 作者 | Kimura, Yukihiro; Nojima, Shingo; Nakata, Kazuna; Yamashita, Takuya; Wang, Xiang-Ping; Takenaka, Shinji; Akimoto, Seiji6; Kobayashi, Masayuki5; Madigan, Michael T.; Wang-Otomo, Zheng-Yu |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
 |
| 出版日期 | 2021 |
| 卷号 | 1862期号:11 |
| 关键词 | Bacteriochlorophyll b-containing light harvesting 1 reaction center Halorhodospira halochloris Interconvertible LH1 Q(y) shift Purple photosynthetic bacteria Triple extremophile |
| ISSN号 | 0005-2728 |
| DOI | 10.1016/j.bbabio.2021.148473 |
| 文献子类 | Article |
| 英文摘要 | Halorhodospira (Hlr.) halochloris is a unique phototrophic purple bacterium because it is a triple extremophile-the organism is thermophilic, alkalophilic, and halophilic. The most striking photosynthetic feature of Hlr. halochloris is that the bacteriochlorophyll (BChl) b-containing core light-harvesting (LH1) complex surrounding its reaction center (RC) exhibits its LH1 Q(y) absorption maximum at 1016 nm, which is the lowest transition energy among phototrophic organisms. Here we report that this extraordinarily red-shifted LH1 Q(y) band of Hlr. halochloris exhibits interconvertible spectral shifts depending on the electrostatic charge distribution around the BChl b molecules. The 1016 nm band of the Hlr. halochloris LH1-RC complex was blue-shifted to 958 nm upon desalting or pH decrease but returned to its original position when supplemented with salts or pH increase. Resonance Raman analysis demonstrated that these interconvertible spectral shifts are not associated with the strength of hydrogen-bonding interactions between BChl b and LH1 polypeptides. Furthermore, circular dichroism signals for the LH1 Q(y) transition of Hlr. halochloris appeared with a positive sign (as in BChl b-containing Blastochloris species) and opposite those of BChl a-containing purple bacteria, possibly due to a combined effect of slight differences in the transition dipole moments between BChl a and BChl b and in the interactions between adjacent BChls in their assembled state. Based on these findings and LH1 amino acid sequences, it is proposed that Hlr. halochloris evolved its unique and tunable light-harvesting system with electrostatic charges in order to carry out photosynthesis and thrive in its punishing hypersaline and alkaline habitat. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 出版地 | AMSTERDAM |
| 电子版国际标准刊号 | 1879-2650 |
| WOS关键词 | BACTERIOCHLOROPHYLL-PROTEIN COMPLEXES ; CIRCULAR-DICHROISM SPECTRA ; LIGHT-HARVESTING COMPLEX ; ECTOTHIORHODOSPIRA-HALOCHLORIS ; SULFUR BACTERIUM ; SP-NOV ; RHODOPSEUDOMONAS-VIRIDIS ; SPECTROSCOPIC PROPERTIES ; STRUCTURAL BASIS ; CORE COMPLEX |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000687486200001 |
| 出版者 | ELSEVIER |
| 资助机构 | Ministry of Education, Culture, Sports, Science and Technology of Japan [19K06563] ; JSPS KAKENHI from Ministry of Education, Culture, Sports, Science and Technology of Japan [JP20H05101, JP16H04174, JP18H05153, JP20H05086, JP20H02856] ; National Natural Science Foundation of China [32070264] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26743]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Ibaraki Univ, Fac Sci, Mito, Ibaraki 3108512, Japan 2.Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Nada Ku, Kobe, Hyogo 6578501, Japan 3.Madigan, Michael T.] Southern Illinois Univ, Dept Microbiol, Carbondale, IL 62901 USA 4.Ariake Natl Coll Technol, Fukuoka 8368585, Japan 5.Kobe Univ, Grad Sch Sci, Dept Sci, Nada Ku, Kobe, Hyogo 6578501, Japan 6.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kimura, Yukihiro,Nojima, Shingo,Nakata, Kazuna,et al. Electrostatic charge controls the lowest LH1 Q(y) transition energy in the triply extremophilic purple phototrophic bacterium, Halorhodospira halochloris[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2021,1862(11). |
| APA | Kimura, Yukihiro.,Nojima, Shingo.,Nakata, Kazuna.,Yamashita, Takuya.,Wang, Xiang-Ping.,...&Yu, Long-Jiang.(2021).Electrostatic charge controls the lowest LH1 Q(y) transition energy in the triply extremophilic purple phototrophic bacterium, Halorhodospira halochloris.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1862(11). |
| MLA | Kimura, Yukihiro,et al."Electrostatic charge controls the lowest LH1 Q(y) transition energy in the triply extremophilic purple phototrophic bacterium, Halorhodospira halochloris".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1862.11(2021). |
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来源：植物研究所
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