The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis
文献类型：期刊论文
| 作者 | Zhang, Jing5,6; Bai, Zechen5; Ouyang, Min4; Xu, Xiumei6; Xiong, Haibo5; Wang, Qiang6; Grimm, Bernhard3; Rochaix, Jean-David; Zhang, Lixin6 |
| 刊名 | EMBO JOURNAL
 |
| 出版日期 | 2021 |
| 卷号 | 40期号:13 |
| ISSN号 | 0261-4189 |
| 关键词 | assembly chloroplast evolution iron donor iron– sulfur cluster |
| DOI | 10.15252/embj.2020106742 |
| 文献子类 | Article |
| 英文摘要 | Fe-S clusters are ancient, ubiquitous and highly essential prosthetic groups for numerous fundamental processes of life. The biogenesis of Fe-S clusters is a multistep process including iron acquisition, sulfur mobilization, and cluster formation. Extensive studies have provided deep insights into the mechanism of the latter two assembly steps. However, the mechanism of iron utilization during chloroplast Fe-S cluster biogenesis is still unknown. Here we identified two Arabidopsis DnaJ proteins, DJA6 and DJA5, that can bind iron through their conserved cysteine residues and facilitate iron incorporation into Fe-S clusters by interactions with the SUF (sulfur utilization factor) apparatus through their J domain. Loss of these two proteins causes severe defects in the accumulation of chloroplast Fe-S proteins, a dysfunction of photosynthesis, and a significant intracellular iron overload. Evolutionary analyses revealed that DJA6 and DJA5 are highly conserved in photosynthetic organisms ranging from cyanobacteria to higher plants and share a strong evolutionary relationship with SUFE1, SUFC, and SUFD throughout the green lineage. Thus, our work uncovers a conserved mechanism of iron utilization for chloroplast Fe-S cluster biogenesis. |
| 学科主题 | Biochemistry & Molecular Biology ; Cell Biology |
| 电子版国际标准刊号 | 1460-2075 |
| 出版地 | HOBOKEN |
| WOS关键词 | ARABIDOPSIS-THALIANA ; OXIDATIVE STRESS ; DEFICIENCY RESPONSES ; SCAFFOLD PROTEIN ; GENE-EXPRESSION ; 4FE-4S CLUSTERS ; MATURATION ; SYSTEM ; HOMEOSTASIS ; FERRITINS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000640181700001 |
| 资助机构 | National Key Research and Development Program [2020YFA0907600] ; 111 Project [D16014] ; Outstanding Talents Fund of Henan University, China |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26783]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Univ Geneva, Dept Plant Biol, Geneva, Switzerland 2.Humboldt Univ, Inst Biol Plant Physiol, Berlin, Germany 3.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adapt & Improvement, Kaifeng, Peoples R China 6.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing, Peoples R China 7.Univ Geneva, Dept Mol Biol, Geneva, Switzerland |
| 推荐引用方式 GB/T 7714 | Zhang, Jing,Bai, Zechen,Ouyang, Min,et al. The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis[J]. EMBO JOURNAL,2021,40(13). |
| APA | Zhang, Jing.,Bai, Zechen.,Ouyang, Min.,Xu, Xiumei.,Xiong, Haibo.,...&Zhang, Lixin.(2021).The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis.EMBO JOURNAL,40(13). |
| MLA | Zhang, Jing,et al."The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis".EMBO JOURNAL 40.13(2021). |
入库方式： OAI收割
来源：植物研究所
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