中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 植物研究所
  3. 中国科学院植物研究所
  4. 中科院光生物学重点实验室
Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus

文献类型：期刊论文

作者Huang, Guoqiang; Xiao, Yanan3; Pi, Xiong; Zhao, Liang; Zhu, Qingjun3; Wang, Wenda; Kuang, Tingyun; Han, Guangye; Sui, Sen-Fang; Shen, Jian-Ren
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2021
卷号118期号:5
关键词photosystem II cryo-EM assembly repair Psb27
ISSN号0027-8424
DOI10.1073/pnas.2018053118
文献子类Article
英文摘要Photosystem II (PSII) is a multisubunit pigment-protein complex and catalyzes light-driven water oxidation, leading to the conversion of light energy into chemical energy and the release of molecular oxygen. Psb27 is a small thylakoid lumen-localized protein known to serve as an assembly factor for the biogenesis and repair of the PSII complex. The exact location and binding fashion of Psb27 in the intermediate PSII remain elusive. Here, we report the structure of a dimeric Psb27-PSII complex purified from a psbV deletion mutant (Delta PsbV) of the cyanobacterium Thermosynechococcus vulcanus, solved by cryo-electron microscopy. Our structure showed that Psb27 is associated with CP43 at the luminal side, with specific interactions formed between Helix 2 and Helix 3 of Psb27 and a loop region between Helix 3 and Helix 4 of CP43 (loop C) as well as the large, lumen-exposed and hydrophilic E-loop of CP43. The binding of Psb27 imposes some conflicts with the N-terminal region of PsbO and also induces some conformational changes in CP43, CP47, and D2. This makes PsbO unable to bind in the Psb27-PSII. Conformational changes also occurred in D1, PsbE, PsbF, and PsbZ; this, together with the conformational changes occurred in CP43, CP47, and D2, may prevent the binding of PsbU and induce dissociation of PsbJ. This structural information provides important insights into the regulation mechanism of Psb27 in the biogenesis and repair of PSII.
学科主题Multidisciplinary Sciences
出版地WASHINGTON
WOS关键词EVOLVING PHOTOSYSTEM-II ; SYNECHOCYSTIS SP PCC-6803 ; PSB27 ASSEMBLY FACTOR ; BINDING-PROTEIN CP43 ; EXTRINSIC PROTEINS ; CRYSTAL-STRUCTURE ; THERMOPHILIC CYANOBACTERIUM ; FUNCTIONAL-PROPERTIES ; ARABIDOPSIS-THALIANA ; DIRECTED MUTAGENESIS
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000615345200035
出版者NATL ACAD SCIENCES
资助机构National Key R&D Program of China [2017YFA0503700, 2016YFA0501101, 2017YFA0504600, 2019YFA0906300] ; National Natural Science Foundation of China [31470339] ; Strategic Priority Research Program of Chinese Academy of Science (CAS) [XDB17000000] ; CAS Key Research program for Frontier Science [QYZDY-SSW-SMC003] ; Youth Innovation Promotion Association of CAS [2020081]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26805]  
专题中科院光生物学重点实验室
作者单位1.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China
2.Tsinghua Univ, Frontier Res Ctr Biol Struct, Sch Life Sci, Beijing 100084, Peoples R China
3.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100084, Peoples R China
6.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama 7008530, Japan
7.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan
推荐引用方式
GB/T 7714		 Huang, Guoqiang,Xiao, Yanan,Pi, Xiong,et al. Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2021,118(5).
APA Huang, Guoqiang.,Xiao, Yanan.,Pi, Xiong.,Zhao, Liang.,Zhu, Qingjun.,...&Shen, Jian-Ren.(2021).Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,118(5).
MLA Huang, Guoqiang,et al."Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 118.5(2021).

入库方式： OAI收割

来源：植物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。