A transporter Slr1512 involved in bicarbonate and pH-dependent acclimation mechanism to high light stress in Synechocystis sp. PCC 6803
文献类型：期刊论文
| 作者 | Xie, Yaru3,4,5; Chen, Lei3,4,5; Sun, Tao1; Jiang, Jingjing6; Tian, Lijin6 ; Cui, Jinyu3,4,5; Zhang, Weiwen1,3,4,5
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| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
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| 出版日期 | 2021 |
| 卷号 | 1862期号:1 |
| 关键词 | Cyanobacteria Synechocystis sp. PCC 6803 High light stress Bicarbonate transport PSII electron transport Transcriptomics |
| ISSN号 | 0005-2728 |
| DOI | 10.1016/j.bbabio.2020.148336 |
| 文献子类 | Article |
| 英文摘要 | High light (HL) exposure leads to photoinhibition and excess accumulation of toxic reactive oxygen species (ROS) in photosynthetic organisms, negatively impacting the global primary production. In this study, by screening a mutant library, a gene related with bicarbonate transport, slr1.512, was found involved in HL acclimation in model cyanobacterium Synechocystis sp. PCC 6803. Comparative growth analysis showed that the slr1.512 knockout mutant dramatically enhanced the tolerance of Synechocystis towards long-term HL stress (200 mu mol photons m(-2) s (-1)) than the wild type, achieving an enhanced growth by similar to 1.95-folds after 10 d. The phenotype differences between Delta slr1.512 and the wild type were analyzed via absorption spectrum and chlorophyll a content measurement. In addition, the accessible bicarbonate controlled by slr1.512 and decreased PSII activity were demonstrated, and they were found to be the key factors affecting the tolerance of Synechocystis against HL stress. Further analysis confirmed that intracellular bicarbonate can significantly affect the activity of photosystem II, leading to the altered accumulation of toxic ROS under HL. Finally, a comparative transcriptomics was applied to determine the differential responses to HL between Delta slr1.512 and the wild type. This work provides useful insights to long-term acclimation mechanisms towards HL and valuable information to guide the future tolerance engineering of cyanobacteria against HL. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 出版地 | AMSTERDAM |
| 电子版国际标准刊号 | 1879-2650 |
| WOS关键词 | SINGLET OXYGEN PRODUCTION ; ELONGATION-FACTOR-G ; PHOTOSYSTEM-II ; GENE-EXPRESSION ; TRANSCRIPTIONAL REGULATOR ; OCEAN ACIDIFICATION ; RANDOM MUTAGENESIS ; HYDROGEN-PEROXIDE ; OXIDATIVE STRESS ; UV-B |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000601394200027 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China [31770035, 31972931, 91751102, 31770100, 31901017, 31901016, 21621004, 1180040312] ; National Key Research and Development Program of China [2019YFA0904600, 2018YFA0903600, 2020YFA0906800, 2018YFA0903000] ; Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project [TSBICIP-KJGG-007] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26820]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 3.Minist Educ China, Frontier Sci Ctr Synthet Biol, Tianjin 300072, Peoples R China 4.Minist Educ China, Key Lab Syst Bioengn, Tianjin 300072, Peoples R China 5.Tianjin Univ, Sch Chem Engn & Technol, Lab Synthet Microbiol, Tianjin 300072, Peoples R China 6.Tianjin Univ, Ctr Biosafety Res & Strategy, Tianjin 300072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Yaru,Chen, Lei,Sun, Tao,et al. A transporter Slr1512 involved in bicarbonate and pH-dependent acclimation mechanism to high light stress in Synechocystis sp. PCC 6803[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2021,1862(1). |
| APA | Xie, Yaru.,Chen, Lei.,Sun, Tao.,Jiang, Jingjing.,Tian, Lijin.,...&Zhang, Weiwen.(2021).A transporter Slr1512 involved in bicarbonate and pH-dependent acclimation mechanism to high light stress in Synechocystis sp. PCC 6803.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1862(1). |
| MLA | Xie, Yaru,et al."A transporter Slr1512 involved in bicarbonate and pH-dependent acclimation mechanism to high light stress in Synechocystis sp. PCC 6803".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1862.1(2021). |
入库方式： OAI收割
来源：植物研究所
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