中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 植物研究所
  3. 中国科学院植物研究所
  4. 中科院北方资源植物重点实验室
GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response

文献类型：期刊论文

作者Wang, Zemin1,2; Wong, Darren Chern Jan3; Wang, Yi1,2; Xu, Guangzhao1,2; Ren, Chong2; Liu, Yanfei1,2; Kuang, Yangfu1,2; Fan, Peige2,4; Li, Shaohua2; Xin, Haiping5,6
刊名PLANT PHYSIOLOGY
出版日期2021
卷号186期号:3页码:1660-1678
ISSN号0032-0889
DOI10.1093/plphys/kiab142
文献子类Article
英文摘要Cultivated grapevine (Vitis) is a highly valued horticultural crop, and cold stress affects its growth and productivity. Wild Amur grape (Vitis amurensis) PAT1 (Phytochrome A signal transduction 1, VaPAT1) is induced by low temperature, and ectopic expression of VaPAT1 enhances cold tolerance in Arabidopsis (Arabidopsis thaliana). However, little is known about the molecular mechanism of VaPAT1 during the cold stress response in grapevine. Here, we confirmed the overexpression of VaPAT1 in transformed grape calli enhanced cold tolerance. Yeast two-hybrid and bimolecular fluorescence complementation assays highlighted an interaction between VaPAT1 with INDETERMINATE-DOMAIN 3 (VaIDD3). A role of VaIDD3 in cold tolerance was also indicated. Transcriptome analysis revealed VaPAT1 and VaIDD3 overexpression and cold treatment coordinately modulate the expression of stress-related genes including lipoxygenase 3 (LOX3), a gene encoding a key jasmonate biosynthesis enzyme. Co-expression network analysis indicated LOX3 might be a downstream target of VaPAT1. Both electrophoretic mobility shift and dual luciferase reporter assays showed the VaPAT1-IDD3 complex binds to the IDD-box (AGACAAA) in the VaLOX3 promoter to activate its expression. Overexpression of both VaPAT1 and VaIDD3 increased the transcription of VaLOX3 and JA levels in transgenic grape calli. Conversely, VaPAT1-SRDX (dominant repression) and CRISPR/Cas9-mediated mutagenesis of PAT1-ED causing the loss of the C-terminus in grape calli dramatically prohibited the accumulation of VaLOX3 and JA levels during cold treatment. Together, these findings point to a pivotal role of VaPAT1 in the cold stress response in grape by regulating JA biosynthesis.
学科主题Plant Sciences
出版地CARY
电子版国际标准刊号1532-2548
WOS关键词GENE-EXPRESSION ; SIGNAL-TRANSDUCTION ; VITIS-AMURENSIS ; SALICYLIC-ACID ; DNA-BINDING ; TOLERANCE ; PROTEIN ; DROUGHT ; FAMILY ; OVEREXPRESSION
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000674744100030
出版者OXFORD UNIV PRESS INC
资助机构National Key Research and Development Program [2019YFD1002501] ; National Science Foundation of China [32025032]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26360]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Innovat Acad Seed Design, Beijing 100093, Peoples R China
3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
4.Australian Natl Univ, Res Sch Biol, Dept Ecol & Evolut, Acton, ACT 2601, Australia
5.China Wine Ind Technol Inst, Yinchuan 750021, Ningxia, Peoples R China
6.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China
7.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Wuhan 430074, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Zemin,Wong, Darren Chern Jan,Wang, Yi,et al. GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response[J]. PLANT PHYSIOLOGY,2021,186(3):1660-1678.
APA Wang, Zemin.,Wong, Darren Chern Jan.,Wang, Yi.,Xu, Guangzhao.,Ren, Chong.,...&Liang, Zhenchang.(2021).GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response.PLANT PHYSIOLOGY,186(3),1660-1678.
MLA Wang, Zemin,et al."GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response".PLANT PHYSIOLOGY 186.3(2021):1660-1678.

入库方式： OAI收割

来源：植物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。