Enzymatic basis for stepwise C-glycosylation in the formation of flavonoid di-C-glycosides in sacred lotus (Nelumbo nucifera Gaertn.)
文献类型：期刊论文
| 作者 | Feng, Cheng-Yong; Li, Shan-Shan; Taguchi, Goro2; Wu, Qian; Yin, Dan-Dan; Gu, Zhao-Yu; Wu, Jie9; Xu, Wen-Zhong; Liu, Cheng; Wang, Liang-Sheng |
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2021 |
| 卷号 | 106期号:2页码:351-365 |
| ISSN号 | 0960-7412 |
| 关键词 | 2‐ hydroxyflavanone C‐ glucosyltransferase C‐ arabinosyltransferase C‐ xylosyltransferase flavonoid C‐ glycosides Nelumbo nucifera sacred lotus |
| DOI | 10.1111/tpj.15168 |
| 文献子类 | Article |
| 英文摘要 | Lotus plumule, the embryo of the seed of the sacred lotus (Nelumbo nucifera), contains a high accumulation of secondary metabolites including flavonoids and possesses important pharmaceutical value. Flavonoid C-glycosides, which accumulate exclusively in lotus plumule, have attracted considerable attention in recent decades due to their unique chemical structure and special bioactivities. As well as mono-C-glycosides, lotus plumule also accumulates various kinds of di-C-glycosides by mechanisms which are as yet unclear. In this study we identified two C-glycosyltransferase (CGT) genes by mining sacred lotus genome data and provide in vitro and in planta evidence that these two enzymes (NnCGT1 and NnCGT2, also designated as UGT708N1 and UGT708N2, respectively) exhibit CGT activity. Recombinant UGT708N1 and UGT708N2 can C-glycosylate 2-hydroxyflavanones and 2-hydroxynaringenin C-glucoside, forming flavone mono-C-glycosides and di-C-glycosides, respectively, after dehydration. In addition, the above reactions were successfully catalysed by cell-free extracts from tobacco leaves transiently expressing NnCGT1 or NnCGT2. Finally, enzyme assays using cell-free extracts of lotus plumule suggested that flavone di-C-glycosides (vicenin-1, vicenin-3, schaftoside and isoschaftoside) are biosynthesized through sequentially C-glucosylating and C-arabinosylating/C-xylosylating 2-hydroxynaringenin. Taken together, our results provide novel insights into the biosynthesis of flavonoid di-C-glycosides by proposing a new biosynthetic pathway for flavone C-glycosides in N. nucifera and identifying a novel uridine diphosphate-glycosyltransferase (UGT708N2) that specifically catalyses the second glycsosylation, C-arabinosylating and C-xylosylating 2-hydroxynaringenin C-glucoside. |
| 学科主题 | Plant Sciences |
| 电子版国际标准刊号 | 1365-313X |
| 出版地 | HOBOKEN |
| WOS关键词 | FUNCTIONAL-CHARACTERIZATION ; PHYLOGENETIC ANALYSIS ; GLYCOSYLTRANSFERASES ; IDENTIFICATION ; BIOSYNTHESIS ; ANTIOXIDANT ; GLUCOSYLTRANSFERASE ; ISOSCHAFTOSIDE ; GLUCOSYLATION ; PUERARIN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000636802200001 |
| 资助机构 | Biological Resources Programme, Chinese Academy of Sciences [KFJ-BRP-007] ; Hebei Provincial Department of Science and Technology [18822911D] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26434]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China 2.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China 4.Shinshu Univ, Fac Text Sci & Technol, Dept Appl Biol, 3-15-1 Tokida, Ueda, Nagano 3868567, Japan 5.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 6.Univ Chinese Acad Sci, Coll Agr, Beijing 100049, Peoples R China 7.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 8.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 9.China Agr Univ, Dept Ornamental Hort, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Cheng-Yong,Li, Shan-Shan,Taguchi, Goro,et al. Enzymatic basis for stepwise C-glycosylation in the formation of flavonoid di-C-glycosides in sacred lotus (Nelumbo nucifera Gaertn.)[J]. PLANT JOURNAL,2021,106(2):351-365. |
| APA | Feng, Cheng-Yong.,Li, Shan-Shan.,Taguchi, Goro.,Wu, Qian.,Yin, Dan-Dan.,...&Wang, Liang-Sheng.(2021).Enzymatic basis for stepwise C-glycosylation in the formation of flavonoid di-C-glycosides in sacred lotus (Nelumbo nucifera Gaertn.).PLANT JOURNAL,106(2),351-365. |
| MLA | Feng, Cheng-Yong,et al."Enzymatic basis for stepwise C-glycosylation in the formation of flavonoid di-C-glycosides in sacred lotus (Nelumbo nucifera Gaertn.)".PLANT JOURNAL 106.2(2021):351-365. |
入库方式： OAI收割
来源：植物研究所
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