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PeMetR-mediated sulfur assimilation is essential for virulence and patulin biosynthesis in Penicillium expansum

文献类型：期刊论文

作者Chen, Yong; Zhang, Zhanquan2; Li, Boqiang2; Tian, Shiping2,3
刊名ENVIRONMENTAL MICROBIOLOGY
出版日期2021
卷号23期号:9页码:5555-5568
ISSN号1462-2912
DOI10.1111/1462-2920.15704
文献子类Article
英文摘要Penicillium expansum, as the causal agent of blue mould and a main producer of mycotoxin patulin, is a global concern for economic and food safety. To date, the nutritional requirements of the pathogen during infection and patulin biosynthesis are poorly understood. Here, we genetically characterized the role of the bZIP transcription factor PeMetR in sulfur metabolism, virulence and patulin biosynthesis of P. expansum. The PeMetR regulator is crucial for normal germination and growth on inorganic S-sources but dispensable for utilization of organic S-sources. Accordingly, it is involved in regulating the expression of genes in sulfur assimilation pathway rather than methionine metabolic processes. Disruption of PeMetR resulted in a complete loss of virulence on various fruits. Additionally, the mutant showed a remarkably reduced ability to produce patulin. Exogenous methionine could partially or completely rescue the impaired phenotypes of the mutant. Inactivation of the sulfur assimilation pathway genes, PesA, PesB, PesC, PesF, generated growth, virulence and patulin production defects similar to those of Delta PeMetR. Overall, our study provides evidence that PeMetR-mediated sulfur assimilation is essential for growth and infection and shows for the first time that regulation of sulfur assimilation affects biosynthesis of an important mycotoxin patulin in P. expansum.
学科主题Microbiology
出版地HOBOKEN
电子版国际标准刊号1462-2920
WOS关键词AREA TRANSCRIPTION FACTOR ; CONTAINING AMINO-ACIDS ; ASPERGILLUS-NIDULANS ; NITROGEN-METABOLISM ; REGULATORY GENE ; NEUROSPORA-CRASSA ; PROTEIN ; GROWTH ; EXPRESSION ; PATHOGENICITY
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000683604100001
出版者WILEY
资助机构National Natural Science Foundation of China [31930086, 32002099, 32072273] ; Youth Innovation Promotion Association, CAS [Y201919] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA26040202] ; China Postdoctoral Science Foundation [2020M670523]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26468]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
3.Minist Agr, Key Lab Postharvest Handing Fruits, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Yong,Zhang, Zhanquan,Li, Boqiang,et al. PeMetR-mediated sulfur assimilation is essential for virulence and patulin biosynthesis in Penicillium expansum[J]. ENVIRONMENTAL MICROBIOLOGY,2021,23(9):5555-5568.
APA Chen, Yong,Zhang, Zhanquan,Li, Boqiang,&Tian, Shiping.(2021).PeMetR-mediated sulfur assimilation is essential for virulence and patulin biosynthesis in Penicillium expansum.ENVIRONMENTAL MICROBIOLOGY,23(9),5555-5568.
MLA Chen, Yong,et al."PeMetR-mediated sulfur assimilation is essential for virulence and patulin biosynthesis in Penicillium expansum".ENVIRONMENTAL MICROBIOLOGY 23.9(2021):5555-5568.

入库方式： OAI收割

来源：植物研究所

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