N-6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner
文献类型：期刊论文
| 作者 | Zhou, Leilei1; Tang, Renkun1; Li, Xiaojing1; Tian, Shiping1; Li, Bingbing2; Qin, Guozheng1 |
| 刊名 | GENOME BIOLOGY
 |
| 出版日期 | 2021 |
| 卷号 | 22期号:1 |
| ISSN号 | 1474-760X |
| 关键词 | m(6)A methylation m(6)A methyltransferase Abscisic acid mRNA stability Translation efficiency Strawberry Fruit ripening |
| DOI | 10.1186/s13059-021-02385-0 |
| 文献子类 | Article |
| 英文摘要 | Background Epigenetic mark such as DNA methylation plays pivotal roles in regulating ripening of both climacteric and non-climacteric fruits. However, it remains unclear whether mRNA m(6)A methylation, which has been shown to regulate ripening of the tomato, a typical climacteric fruit, is functionally conserved for ripening control among different types of fruits. Results Here we show that m(6)A methylation displays a dramatic change at ripening onset of strawberry, a classical non-climacteric fruit. The m(6)A modification in coding sequence (CDS) regions appears to be ripening-specific and tends to stabilize the mRNAs, whereas m(6)A around the stop codons and within the 3 ' untranslated regions is generally negatively correlated with the abundance of associated mRNAs. We identified thousands of transcripts with m(6)A hypermethylation in the CDS regions, including those of NCED5, ABAR, and AREB1 in the abscisic acid (ABA) biosynthesis and signaling pathway. We demonstrate that the methyltransferases MTA and MTB are indispensable for normal ripening of strawberry fruit, and MTA-mediated m(6)A modification promotes mRNA stability of NCED5 and AREB1, while facilitating translation of ABAR. Conclusion Our findings uncover that m(6)A methylation regulates ripening of the non-climacteric strawberry fruit by targeting the ABA pathway, which is distinct from that in the climacteric tomato fruit. |
| 学科主题 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 出版地 | LONDON |
| WOS关键词 | NUCLEAR-RNA ; NEGATIVE REGULATOR ; PROTEIN-KINASES ; CELL FATE ; METHYLATION ; REVEALS ; TRANSLATION ; DEMETHYLASE ; N6-METHYLADENOSINE ; RECEPTORS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | BMC |
| WOS记录号 | WOS:000657355600001 |
| 资助机构 | National Key Research and Development Program [2018YFD1000200] ; National Natural Science Foundation of China [31925035, 31930086, 31572174] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26527]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Agr Univ, Coll Hort, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Leilei,Tang, Renkun,Li, Xiaojing,et al. N-6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner[J]. GENOME BIOLOGY,2021,22(1). |
| APA | Zhou, Leilei,Tang, Renkun,Li, Xiaojing,Tian, Shiping,Li, Bingbing,&Qin, Guozheng.(2021).N-6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner.GENOME BIOLOGY,22(1). |
| MLA | Zhou, Leilei,et al."N-6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner".GENOME BIOLOGY 22.1(2021). |
入库方式： OAI收割
来源：植物研究所
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