Genome Wide Analysis of GH Gene Family Reveals Vvgh9 Positively Regulates Sugar Accumulation under Low Sugar Content in Grape
文献类型：期刊论文
| 作者 | Xu, Guangzhao1; Wang, Yi; Ren, Chong; Fan, Peige; Kuang, Yangfu1; Wang, Yue1; Liang, Zhenchang |
| 刊名 | HORTICULTURAE
 |
| 出版日期 | 2021 |
| 卷号 | 7期号:11 |
| 关键词 | grape sugar VvGH9 transcriptomic analysis overexpression |
| DOI | 10.3390/horticulturae7110453 |
| 文献子类 | Article |
| 英文摘要 | Sugar content directly affects grape (Vitis vinifera L.) berry quality and the resulting wine. Therefore, it is of great importance to study and explore novel genes that affect sugar accumulation in grapes. Glycosyl hydrolases (GHs) are key enzymes hydrolyzing polysaccharides into monosaccharides and play important roles in the regulation of carbohydrate metabolism. Nevertheless, the impact of GHs on the regulation of sugar accumulation in plants has rarely been investigated. In this study, we identified 11 putative GH genes in grapevines by phylogeny analysis. RNA-seq and quantitative real-time PCR results demonstrated that the expression level of VvGH9 was higher during the fruit set stage, which had lower sugar content than the veraison and ripe stages. Treatment of grape berries with exogenous sugar two weeks before veraison revealed that VvGH9 was rapidly induced by sucrose, fructose, and glucose. When '41B' calli was treated with different concentrations of glucose, VvGH9 expression increased at first and then decreased with the increase of glucose concentration. Overexpression of VvGH9 in grape calli and tomatoes also confirmed that this gene could contribute to sugar accumulation. All the above results demonstrated that VvGH9 promotes sugar accumulation under low sugar content in plants. |
| 学科主题 | Horticulture |
| 出版地 | BASEL |
| 电子版国际标准刊号 | 2311-7524 |
| WOS关键词 | GLYCOSYL HYDROLASES ; STRESS ; IDENTIFICATION ; GLUCOSIDASE ; ENZYMES ; GROWTH |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000724360700001 |
| 出版者 | MDPI |
| 资助机构 | Strategic Priority Research Program of Chinese Academy of Sciences [XDA24030404-3] ; National Key Research and Development Program of China [2018YFD1000105] ; China-Africa Center for Research and Education, Chinese Academy of Sciences [151111KYSB20200017] ; Alliance of International Science Organizations [ANSO-CR-PP-2020-04] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26553]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Guangzhao,Wang, Yi,Ren, Chong,et al. Genome Wide Analysis of GH Gene Family Reveals Vvgh9 Positively Regulates Sugar Accumulation under Low Sugar Content in Grape[J]. HORTICULTURAE,2021,7(11). |
| APA | Xu, Guangzhao.,Wang, Yi.,Ren, Chong.,Fan, Peige.,Kuang, Yangfu.,...&Liang, Zhenchang.(2021).Genome Wide Analysis of GH Gene Family Reveals Vvgh9 Positively Regulates Sugar Accumulation under Low Sugar Content in Grape.HORTICULTURAE,7(11). |
| MLA | Xu, Guangzhao,et al."Genome Wide Analysis of GH Gene Family Reveals Vvgh9 Positively Regulates Sugar Accumulation under Low Sugar Content in Grape".HORTICULTURAE 7.11(2021). |
入库方式： OAI收割
来源：植物研究所
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