Transcriptomic analysis of grapevine Dof transcription factor gene family in response to cold stress and functional analyses of the VaDof17d gene
文献类型：期刊论文
| 作者 | Wang, Zemin2,3; Wang, Yi2,3; Tong, Qian2,3; Xu, Guangzhao2,3; Xu, Meilong1,2,3; Li, Huayang2,3; Fan, Peige2,5; Li, Shaohua2,3 ; Liang, Zhenchang2,4
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| 刊名 | PLANTA
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| 出版日期 | 2021 |
| 卷号 | 253期号:2 |
| 关键词 | Cold stress CRISPR Cas9 Grapevine dof family Phylogenetic analysis Raffinose Transcriptomic analysis |
| ISSN号 | 0032-0935 |
| DOI | 10.1007/s00425-021-03574-8 |
| 文献子类 | Article |
| 英文摘要 | Main conclusionDof genes enhance cold tolerance in grapevine and VaDof17d is tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides.AbstractDNA-binding with one finger (Dof) proteins comprise a large family that plays important roles in the regulation of abiotic stresses. No in-depth analysis of Dof genes has been performed in the grapevine. In this study, we analyzed a total of 25 putative Dof genes in grapevine at genomic and transcriptomic levels, compiled expression profiles of 11 selected VaDof genes under cold stress and studied the potential function of the VaDof17d gene in grapevine calli. The 25 Dof proteins can be classified into four phylogenetic groups. RNA-seq and qRT-PCR results demonstrated that a total of 11 VaDof genes responded to cold stress. Comparative mRNA sequencing of 35S::VaDof17d grape calli showed that VaDof17d was tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides (RFOs), as observed by the up-regulation of galactinol synthase (GolS) and raffinose synthase genes. We found that the Dof17d-ED (CRISPR/Cas9-mediated mutagenesis of Dof17d-ED) mutant had low cold tolerance with a decreased RFOs level during cold stress. These results formed the fundamental knowledge for further analysis of the biological roles of Dof genes in the grapevine's adaption to cold stresses. |
| 学科主题 | Plant Sciences |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1432-2048 |
| WOS关键词 | DNA-BINDING PROTEINS ; GENOME-WIDE ANALYSIS ; GALACTINOL SYNTHASE ; FREEZING TOLERANCE ; LOW-TEMPERATURE ; VITIS-VINIFERA ; PLANT ; EXPRESSION ; INVOLVEMENT ; OVEREXPRESSION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000616149400001 |
| 出版者 | SPRINGER |
| 资助机构 | National Key Research and Development Program [2019YFD1002501] ; National Science Foundation of China [32025032] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26565]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.China Wine Ind Technol Inst, Yinchuan 750021, Ningxia, Peoples R China 2.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Innovat Acad Seed Design, Beijing 100093, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Plant Resources, Inst Bot, Innovat Acad Seed Design, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China 6.State Key Lab Seedling Bioengn, Yinchuan 750004, Ningxia, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zemin,Wang, Yi,Tong, Qian,et al. Transcriptomic analysis of grapevine Dof transcription factor gene family in response to cold stress and functional analyses of the VaDof17d gene[J]. PLANTA,2021,253(2). |
| APA | Wang, Zemin.,Wang, Yi.,Tong, Qian.,Xu, Guangzhao.,Xu, Meilong.,...&Liang, Zhenchang.(2021).Transcriptomic analysis of grapevine Dof transcription factor gene family in response to cold stress and functional analyses of the VaDof17d gene.PLANTA,253(2). |
| MLA | Wang, Zemin,et al."Transcriptomic analysis of grapevine Dof transcription factor gene family in response to cold stress and functional analyses of the VaDof17d gene".PLANTA 253.2(2021). |
入库方式： OAI收割
来源：植物研究所
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