Genome-Wide Analysis of the UGT Gene Family and Identification of Flavonoids in Broussonetia papyrifera
文献类型：期刊论文
| 作者 | Wang, Fenfen2; Su, Yalei2; Chen, Naizhi; Shen, Shihua
|
| 刊名 | MOLECULES
 |
| 出版日期 | 2021 |
| 卷号 | 26期号:11 |
| 关键词 | Broussonetia polyphenols flavonoid antioxidant activity BpUGTs |
| DOI | 10.3390/molecules26113449 |
| 文献子类 | Article |
| 英文摘要 | Broussonetia papyrifera is a multifunctional deciduous tree that is both a food and a source of traditional Chinese medicine for both humans and animals. Further analysis of the UGT gene family is of great significance to the utilization of B. papyrifera. The substrates of plant UGT genes include highly diverse and complex chemicals, such as flavonoids and terpenes. In order to deepen our understanding of this family, a comprehensive analysis was performed. Phylogenetic analysis showed that 155 BpUGTs were divided into 15 subgroups. A conserved motif analysis showed that BpUGT proteins in the same subgroups possessed similar motif structures. Tandem duplication was the primary driving force for the expansion of the BpUGT gene family. The global promoter analysis indicated that they were associated with complex hormone regulatory networks and the stress response, as well as the synthesis of secondary metabolites. The expression pattern analysis showed that the expression level of BpUGTs in leaves and roots was higher than that in fruits and stems. Next, we determined the composition and content of flavonoids, the main products of the BpUGT reaction. A total of 19 compounds were isolated and analyzed by UPLC-ESI-MS/MS in 3 species of Broussonetia including B. kazinoki, B. papyrifera, and B. kazinoki x B. papyrifera, and the number of compounds was different in these 3 species. The total flavonoid content and antioxidant capacities of the three species were analyzed respectively. All assays exhibited the same trend: the hybrid paper mulberry showed a higher total flavonoid content, a higher total phenol content and higher antioxidant activity than the other two species. Overall, our study provides valuable information for understanding the function of BpUGTs in the biosynthesis of flavonoids. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 出版地 | BASEL |
| 电子版国际标准刊号 | 1420-3049 |
| WOS关键词 | CHLOROGENIC ACID ; IN-VITRO ; GLYCOSYLTRANSFERASES ; PLANT ; BIOSYNTHESIS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000660362000001 |
| 出版者 | MDPI |
| 资助机构 | National Natural Science Foundation of China [31870247] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26571]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fenfen,Su, Yalei,Chen, Naizhi,et al. Genome-Wide Analysis of the UGT Gene Family and Identification of Flavonoids in Broussonetia papyrifera[J]. MOLECULES,2021,26(11). |
| APA | Wang, Fenfen,Su, Yalei,Chen, Naizhi,&Shen, Shihua.(2021).Genome-Wide Analysis of the UGT Gene Family and Identification of Flavonoids in Broussonetia papyrifera.MOLECULES,26(11). |
| MLA | Wang, Fenfen,et al."Genome-Wide Analysis of the UGT Gene Family and Identification of Flavonoids in Broussonetia papyrifera".MOLECULES 26.11(2021). |
入库方式： OAI收割
来源：植物研究所
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