Ubiquitin-independent proteasome system is required for degradation of Arabidopsis COPPER TRANSPORTER 2
文献类型：期刊论文
| 作者 | Li, Jinjin1; Wang, Weiwei; Yuan, Jinhong1; Xu, Jinyu; He, Lifei; Zhang, Xinying; Zhang, Haiyan |
| 刊名 | PLANT SCIENCE
 |
| 出版日期 | 2021 |
| 卷号 | 304 |
| 关键词 | COPT2 Arabidopsis Vacuolar degradation Proteasome Ubiquitin Vesicle trafficking |
| ISSN号 | 0168-9452 |
| DOI | 10.1016/j.plantsci.2021.110825 |
| 文献子类 | Article |
| 英文摘要 | Plants have evolved sophisticated mechanisms to adjust to deficiency or excess of nutrients. Membrane transport proteins play a central role in nutrient uptake from soil. In Arabidopsis thaliana, the COPPER TRANSPORTOR (COPT) family encodes high-affinity copper transporters. COPT2 is transcriptionally regulated in response to changing levels of cellular copper. However, little is known about whether COPT2 activity is subject to multiple levels of regulation. Here, we showed that the plasma membrane-/endoplasmic reticulum-resident COPT2 protein is degraded in response to excess copper. Confocal microscopy analysis together with pharmacological treatment with a vesicle trafficking inhibitor or vacuolar ATPase inhibitor indicated that copper-mediated downregulation of COPT2 is unlikely to be controlled by endosomal recycling and vacuolar system. However, COPT2 protein is stabilized by proteasome inhibition. Through site-directed mutagenesis, we found that COPT2 cannot be ubiquitinated, and lysine residues at the C-terminus is dispensable for copper-induced degradation of COPT2 but required for copper acquisition. Altogether, our findings reveal that unlike many metal transporters in Arabidopsis, COPT2 is a substrate of ubiquitin-independent proteasomal degradation but not of vacuolar proteases. These findings highlight the mechanistic diversity and complexity of plasma membrane transporter degradation. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | CLARE |
| 电子版国际标准刊号 | 1873-2259 |
| WOS关键词 | PLASMA-MEMBRANE PROTEINS ; ENDOPLASMIC-RETICULUM ; DEFICIENCY RESPONSES ; IRON HOMEOSTASIS ; AUXIN TRANSPORT ; ENDOCYTOSIS ; TRAFFICKING ; EXPRESSION ; ROOT ; ACCUMULATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000626081800003 |
| 出版者 | ELSEVIER IRELAND LTD |
| 资助机构 | National Natural Science Foundation of China [31470292] ; Natural Science Foundation of Tianjin ; [18JCZDJC97900] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26578]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jinjin,Wang, Weiwei,Yuan, Jinhong,et al. Ubiquitin-independent proteasome system is required for degradation of Arabidopsis COPPER TRANSPORTER 2[J]. PLANT SCIENCE,2021,304. |
| APA | Li, Jinjin.,Wang, Weiwei.,Yuan, Jinhong.,Xu, Jinyu.,He, Lifei.,...&Zhang, Haiyan.(2021).Ubiquitin-independent proteasome system is required for degradation of Arabidopsis COPPER TRANSPORTER 2.PLANT SCIENCE,304. |
| MLA | Li, Jinjin,et al."Ubiquitin-independent proteasome system is required for degradation of Arabidopsis COPPER TRANSPORTER 2".PLANT SCIENCE 304(2021). |
入库方式： OAI收割
来源：植物研究所
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