Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy
文献类型：期刊论文
| 作者 | Tang, Ling1; Li, Yang1; Zhong, Cheng3; Deng, Xin; Wang, Xiaohua |
| 刊名 | MEMBRANES
 |
| 出版日期 | 2021 |
| 卷号 | 11期号:10 |
| 关键词 | FLIM computational microscopy phase separation microdomain phytosterol |
| DOI | 10.3390/membranes11100747 |
| 文献子类 | Article |
| 英文摘要 | Local inhomogeneities in lipid composition play a crucial role in the regulation of signal transduction and membrane traffic. This is particularly the case for plant plasma membrane, which is enriched in specific lipids, such as free and conjugated forms of phytosterols and typical phytosphingolipids. Nevertheless, most evidence for microdomains in cells remains indirect, and the nature of membrane inhomogeneities has been difficult to characterize. We used a new push-pull pyrene probe and fluorescence lifetime imaging microscopy (FLIM) combined with all-atom multiscale molecular dynamics simulations to provide a detailed view on the interaction between phospholipids and phytosterol and the effect of modulating cellular phytosterols on membrane-associated microdomains and phase separation formation. Our understanding of the organization principles of biomembranes is limited mainly by the challenge to measure distributions and interactions of lipids and proteins within the complex environment of living cells. Comparing phospholipids/phytosterol compositions typical of liquid-disordered (Ld) and liquid-ordered (Lo) domains, we furthermore show that phytosterols play crucial roles in membrane homeostasis. The simulation work highlights how state-of-the-art modeling alleviates some of the prior concerns and how unrefuted discoveries can be made through a computational microscope. Altogether, our results support the role of phytosterols in the lateral structuring of the PM of plant cells and suggest that they are key compounds for the formation of plant PM microdomains and the lipid-ordered phase. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Physical ; Engineering, Chemical ; Materials Science, Multidisciplinary ; Polymer Science |
| 电子版国际标准刊号 | 2077-0375 |
| 出版地 | BASEL |
| WOS关键词 | X-RAY-DIFFRACTION ; MOLECULAR-DYNAMICS SIMULATIONS ; BREWSTER-ANGLE MICROSCOPY ; PLASMA-MEMBRANE ; LIPID ORDER ; FREE-ENERGY ; ARABIDOPSIS ; CHOLESTEROL ; PHOSPHOLIPIDS ; VISUALIZATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000717013600001 |
| 资助机构 | National Natural Science Foundation of China [3217020622, 31770400, 31771493] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26588]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Wuhan Univ, Dept Chem, Hubei Key Lab Organ & Polymer Optoelect Mat, Wuhan 430072, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Ling,Li, Yang,Zhong, Cheng,et al. Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy[J]. MEMBRANES,2021,11(10). |
| APA | Tang, Ling,Li, Yang,Zhong, Cheng,Deng, Xin,&Wang, Xiaohua.(2021).Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy.MEMBRANES,11(10). |
| MLA | Tang, Ling,et al."Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy".MEMBRANES 11.10(2021). |
入库方式： OAI收割
来源：植物研究所
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