Identification of monocot chimeric jacalin family reveals functional diversity in wheat
文献类型：期刊论文
| 作者 | Ma, Qing-Hu; Han, Jia-Qi |
| 刊名 | PLANTA
 |
| 出版日期 | 2021 |
| 卷号 | 253期号:2 |
| 关键词 | Agglutination Dehydration stress Evolutionary path Disease resistance MCJ family Wheat |
| ISSN号 | 0032-0935 |
| DOI | 10.1007/s00425-020-03548-2 |
| 文献子类 | Article |
| 英文摘要 | Main conclusion 46 monocot chimeric jacalins (MCJs) were mined from wheat genome. They were divided into three subfamilies with the activity of mannose-specific lectins and had effects on dehydration tolerance or disease resistance. Monocot chimeric jacalin (MCJ) is a newly identified subfamily of plant lectins that exclusively exists in Poaceae. The MCJs are modular proteins consisting of a dirigent domain and a jacalin-related lectin domain. Their unique evolution and various functions are not fully understood as only few members of MCJ have so for been investigated. From wheat, 46 MCJs were identified and phylogenetically classified into three subfamilies, in which subfamily I represented the early evolutionary cluster. MCJ genes are evenly distributed among three subgenomes of wheat, indicating that MCJ might be an ancient gene in Poaceae. qRT-PCR analysis showed that TaMCJ1 and TaMCJ2 were mainly expressed in leaves while TaMCJ3 in root tissues. All these TaMCJ genes are JA or ABA inducible. All three proteins exhibited agglutinating activity but different preference to mannose-binding. The overexpression of TaMCJ3 in tobacco increased dehydration tolerance, while TaMCJ1 enhanced wildfire disease resistance. The lignin biosynthetic genes were temporarily induced after pathogen inoculation in transgenic tobacco overexpressing TaMCJ, but the specific association with TaMCJ was not established. This evidence argued against the notion that the dirigent domain in TaMCJ is directly linked with lignin metabolism. Taken together, these results pave the way for a better understanding of the manifold functionality of MCJs and offer important insights to the evolutionary history of MCJ. |
| 学科主题 | Plant Sciences |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1432-2048 |
| WOS关键词 | GLUCOSIDASE-AGGREGATING FACTOR ; GENE-EXPRESSION ; LECTIN DOMAIN ; PROTEIN ; OVEREXPRESSION ; LIGNIN ; PLANTS ; RESISTANCE ; TOLERANCE ; BIOMASS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000608368100003 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China [31870274] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26680]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, 20 Nanxincun, Beijing 100093, Peoples R China 3.[Ma, Qing-Hu |
| 推荐引用方式 GB/T 7714 | Ma, Qing-Hu,Han, Jia-Qi. Identification of monocot chimeric jacalin family reveals functional diversity in wheat[J]. PLANTA,2021,253(2). |
| APA | Ma, Qing-Hu,&Han, Jia-Qi.(2021).Identification of monocot chimeric jacalin family reveals functional diversity in wheat.PLANTA,253(2). |
| MLA | Ma, Qing-Hu,et al."Identification of monocot chimeric jacalin family reveals functional diversity in wheat".PLANTA 253.2(2021). |
入库方式： OAI收割
来源：植物研究所
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