Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum
文献类型：期刊论文
| 作者 | Liu, Guoquan; Zhang, Yan; Gong, Hao; Li, Shan; Pan, Yunrong3; Davis, Christopher3; Jing, Hai-Chun ; Wu, Luguang3; Godwin, Ian D.
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| 刊名 | BIOTECHNOLOGY FOR BIOFUELS
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| 出版日期 | 2021 |
| 卷号 | 14期号:1 |
| 关键词 | Isomaltulose Sorghum Sucrose isomerase Sugar content Renewable energy Photosynthesis Genetic engineering Sugarcane |
| DOI | 10.1186/s13068-021-01907-z |
| 文献子类 | Article |
| 英文摘要 | BackgroundSugar content is critically important in determining sugar crop productivity. However, improvement in sugar content has been stagnant among sugar crops for decades. Sorghum, especially sweet sorghum with high biomass, shown great potential for biofuel, has lower sugar content than sugarcane. To enhance sugar content, the sucrose isomerase (SI) gene, driven by stem-specific promoters (A2 or LSG) with a vacuole-targetted signal peptide, was transformed into the sorghum inbred line (Tx430).ResultsThe study demonstrated that transgenic lines of grain sorghum, containing 50-60% isomaltulose, accumulated up to eightfold (1000 mM) more total sugar than the control Tx430 did (118 mM) in stalks of T-0 generation. Subsequently, the elite engineered lines (A5, and LSG9) were crossed with sweet sorghum (Rio, and R9188). Total sugar contents (over 750 mM), were notably higher in F-1, and F-2 progenies than the control Rio (480 mM). The sugar contents of the engineered lines (over 750 mM), including T-0, T-1, F-1, and F-2, are surprisingly higher than that of the field-grown sugarcane (normal range 600-700 mmol/L). Additionally, analysis of physiological characterization demonstrated that the superior progenies had notably higher rates of photosynthesis, sucrose transportation, and sink strength than the controls.ConclusionsThe genetic engineering approach has dramatically enhanced total sugar content in grain sorghum (T-0, and T-1) and hybrid sorghum (F-1, and F-2), demonstrating that sorghum can accumulate as high or higher sugar content than sugarcane. This research illustrates that the SI gene has enormous potential on improvement of sugar content in sorghum, particularly in hybirds and sweet sorghum. The substantial increase on sugar content would lead to significant financial benefits for industrial utilization. This study could have a substantial impact on renewable bioenergy. More importantly, our results demonstrated that the phenotype of high sugar content is inheritable and shed light on improvement for other sugar crops. |
| 学科主题 | Biotechnology & Applied Microbiology ; Energy & Fuels |
| 出版地 | LONDON |
| 电子版国际标准刊号 | 1754-6834 |
| WOS关键词 | PANTOEA-DISPERSA UQ68J ; SWEET SORGHUM ; ISOMALTULOSE PALATINOSE(R) ; POTATO-TUBERS ; ACCUMULATION ; METABOLISM ; EXPRESSION ; MECHANISMS ; PROMOTERS ; BIOMASS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000625383800002 |
| 出版者 | BMC |
| 资助机构 | Queensland Department of Environment and Science |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26688]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 2.Univ Queensland, Sch Agr & Food Sci, Brisbane, Qld 4072, Australia 3.[Liu, Guoquan; Zhang, Yan; Gong, Hao; Li, Shan; Godwin, Ian D.] Univ Queensland, Ctr Crop Sci, Queensland Alliance Agr & Food Innovat, Brisbane, Qld 4072, Australia |
| 推荐引用方式 GB/T 7714 | Liu, Guoquan,Zhang, Yan,Gong, Hao,et al. Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum[J]. BIOTECHNOLOGY FOR BIOFUELS,2021,14(1). |
| APA | Liu, Guoquan.,Zhang, Yan.,Gong, Hao.,Li, Shan.,Pan, Yunrong.,...&Godwin, Ian D..(2021).Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum.BIOTECHNOLOGY FOR BIOFUELS,14(1). |
| MLA | Liu, Guoquan,et al."Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum".BIOTECHNOLOGY FOR BIOFUELS 14.1(2021). |
入库方式： OAI收割
来源：植物研究所
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