Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola
文献类型：期刊论文
| 作者 | Liu, Guo-Tian; Wang, Bian-Bian; Lecourieux, David; Li, Mei-Jie; Liu, Ming-Bo; Liu, Rui-Qi; Shang, Bo-Xing; Yin, Xiao; Wang, Li-Jun; Lecourieux, Fatma7 |
| 刊名 | HORTICULTURE RESEARCH
 |
| 出版日期 | 2021 |
| 卷号 | 8期号:1 |
| ISSN号 | 2662-6810 |
| DOI | 10.1038/s41438-021-00533-y |
| 文献子类 | Article |
| 英文摘要 | Wild grapevines can show strong resistance to the downy mildew pathogen P. viticola, but the associated mechanisms are poorly described, especially at early stages of infection. Here, we performed comparative proteomic analyses of grapevine leaves from the resistant genotype V. davidii LiuBa-8 (LB) and susceptible V. vinifera Pinot Noir (PN) 12 h after inoculation with P. viticola. By employing the iTRAQ technique, a total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN, respectively. The majority of these DEPs were related to photosynthesis, respiration, cell wall modification, protein metabolism, stress, and redox homeostasis. Compared with PN, LB showed fewer downregulated proteins associated with photosynthesis and more upregulated proteins associated with metabolism. At least a subset of PR proteins (PR10.2 and PR10.3) was upregulated upon inoculation in both genotypes, whereas HSP (HSP70.2 and HSP90.6) and cell wall-related XTH and BXL1 proteins were specifically upregulated in LB and PN, respectively. In the incompatible interaction, ROS signaling was evident by the accumulation of H2O2, and multiple APX and GST proteins were upregulated. These DEPs may play crucial roles in the grapevine response to downy mildew. Our results provide new insights into molecular events associated with downy mildew resistance in grapevine, which may be exploited to develop novel protection strategies against this disease. |
| 学科主题 | Plant Sciences ; Genetics & Heredity ; Horticulture |
| 出版地 | CARY |
| 电子版国际标准刊号 | 2052-7276 |
| WOS关键词 | TRANSGENIC TOBACCO PLANTS ; DEFENSE-RELATED GENES ; VITIS-VINIFERA ; PLASMOPARA-VITICOLA ; DISEASE RESISTANCE ; DOWNY MILDEW ; CELL-WALL ; MOLECULAR CHARACTERIZATION ; PHYTOPHTHORA-INFESTANS ; ENHANCED RESISTANCE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000645936200018 |
| 出版者 | OXFORD UNIV PRESS INC |
| 资助机构 | National Key Research and Development Program of China [2018YFD1000300, 2019YFD1002500] ; National Natural Science Foundation of China [31601716, 31972374] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26692]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Yin, Xiao 2.Liu, Rui-Qi 3.Liu, Ming-Bo 4.Li, Mei-Jie 5.Wang, Bian-Bian 6.[Liu, Guo-Tian 7.Lecourieux, David 8.Northwest A&F Univ, Key Lab Hort Plant Biol & Germplasm Innovat North, Minist Agr, Yangling, Shaanxi, Peoples R China 9.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China 10.Shang, Bo-Xing |
| 推荐引用方式 GB/T 7714 | Liu, Guo-Tian,Wang, Bian-Bian,Lecourieux, David,et al. Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola[J]. HORTICULTURE RESEARCH,2021,8(1). |
| APA | Liu, Guo-Tian.,Wang, Bian-Bian.,Lecourieux, David.,Li, Mei-Jie.,Liu, Ming-Bo.,...&Xu, Yan.(2021).Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola.HORTICULTURE RESEARCH,8(1). |
| MLA | Liu, Guo-Tian,et al."Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola".HORTICULTURE RESEARCH 8.1(2021). |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。