Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis
文献类型：期刊论文
| 作者 | Li, Xiaoxia; Yang, Weiguang2; Jia, Junting1; Zhao, Pincang5; Qi, Dongmei; Chen, Shuangyan; Cheng, Li4; Cheng, Liqin; Liu, Gongshe
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| 刊名 | GENES
 |
| 出版日期 | 2021 |
| 卷号 | 12期号:12 |
| 关键词 | sheepgrass chloroplast salt stress seed germination |
| DOI | 10.3390/genes12121994 |
| 文献子类 | Article |
| 英文摘要 | Sheepgrass is a perennial native grass species in China, and it can tolerate high levels of salt stress with an aggressive and vigorous rhizome system. Many salt-stress-responsive genes have been identified in sheepgrass. In this study, we report the cloning and characterization of a novel salt-induced gene, LcSAIN3 (Leymus chinensis salt-induced 3), from sheepgrass. Expression analysis confirmed that LcSAIN3 was induced by PEG, ABA, and salt treatments, and the expression of LcSAIN3 was significantly increased in salt-tolerant germplasms under salt treatment. Subcellular localization analysis indicated that the GFP-LcSAIN3 protein was mainly localized in the chloroplasts. The heterologous expression of LcSAIN3 in Arabidopsis increased the seed germination rate of transgenic plants under salt, ABA, and mannitol treatments. The seedling survival rate, plant height, and fresh weight of the transgenic plants were higher than those of WT plants under salt stress. The overexpression of LcSAIN3 caused a relatively high accumulation of free proline, enhanced SOD activity, and led to the upregulation of several stress-responsive genes such as AtRD26, AtRD29B, AtSOS1, and AtP5CS1. These results suggest that LcSAIN3 could be a potential target for molecular breeding to improve plants' salt tolerance. |
| 学科主题 | Genetics & Heredity |
| 出版地 | BASEL |
| 电子版国际标准刊号 | 2073-4425 |
| WOS关键词 | TRANSCRIPTION FACTOR GENE ; TRANSGENIC ARABIDOPSIS ; ENHANCES TOLERANCE ; LOW-TEMPERATURE ; DREB GENE ; MYB GENE ; DROUGHT ; COLD ; OVEREXPRESSION ; PROTEIN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000737537700001 |
| 出版者 | MDPI |
| 资助机构 | National Natural Science Foundation of China [32071869] ; Key Projects in Science and Technology of the Inner Mongolia [2021ZD0031] ; Strategic Priority Research Program of the Chinese Academy of Science [XDA26030202] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26700]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Helongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163000, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Inst Hulun Buir Forestry & Grassland Sci Res, Hunlunbeier 021008, Peoples R China 4.Hebei Univ Econ & Business, Coll Management Sci & Engn, Shijiazhuang 050062, Hebei, Peoples R China 5.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Prov Key Lab Crop Germplasm Resources P, Guangzhou 510640, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiaoxia,Yang, Weiguang,Jia, Junting,et al. Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis[J]. GENES,2021,12(12). |
| APA | Li, Xiaoxia.,Yang, Weiguang.,Jia, Junting.,Zhao, Pincang.,Qi, Dongmei.,...&Liu, Gongshe.(2021).Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis.GENES,12(12). |
| MLA | Li, Xiaoxia,et al."Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis".GENES 12.12(2021). |
入库方式： OAI收割
来源：植物研究所
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