Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis
文献类型：期刊论文
| 作者 | Huang, Hui2; Liang, Juan; Tan, Qi; Ou, Linfeng; Li, Xiaolin1; Zhong, Caihong; Huang, Huilin; Moller, Ian Max3; Wu, Xianjin; Song, Songquan4 |
| 刊名 | HORTICULTURE RESEARCH
 |
| 出版日期 | 2021 |
| 卷号 | 8期号:1 |
| ISSN号 | 2662-6810 |
| DOI | 10.1038/s41438-020-00458-y |
| 文献子类 | Article |
| 英文摘要 | Akebia trifoliata subsp. australis is a well-known medicinal and potential woody oil plant in China. The limited genetic information available for A. trifoliata subsp. australis has hindered its exploitation. Here, a high-quality chromosome-level genome sequence of A. trifoliata subsp. australis is reported. The de novo genome assembly of 682.14Mb was generated with a scaffold N50 of 43.11Mb. The genome includes 25,598 protein-coding genes, and 71.18% (485.55Mb) of the assembled sequences were identified as repetitive sequences. An ongoing massive burst of long terminal repeat (LTR) insertions, which occurred similar to 1.0 million years ago, has contributed a large proportion of LTRs in the genome of A. trifoliata subsp. australis. Phylogenetic analysis shows that A. trifoliata subsp. australis is closely related to Aquilegia coerulea and forms a clade with Papaver somniferum and Nelumbo nucifera, which supports the well-established hypothesis of a close relationship between basal eudicot species. The expansion of UDP-glucoronosyl and UDP-glucosyl transferase gene families and beta-amyrin synthase-like genes and the exclusive contraction of terpene synthase gene families may be responsible for the abundant oleanane-type triterpenoids in A. trifoliata subsp. australis. Furthermore, the acyl-ACP desaturase gene family, including 12 stearoyl-acyl-carrier protein desaturase (SAD) genes, has expanded exclusively. A combined transcriptome and fatty-acid analysis of seeds at five developmental stages revealed that homologs of SADs, acyl-lipid desaturase omega fatty acid desaturases (FADs), and oleosins were highly expressed, consistent with the rapid increase in the content of fatty acids, especially unsaturated fatty acids. The genomic sequences of A. trifoliata subsp. australis will be a valuable resource for comparative genomic analyses and molecular breeding. |
| 学科主题 | Plant Sciences ; Genetics & Heredity ; Horticulture |
| 出版地 | CARY |
| 电子版国际标准刊号 | 2052-7276 |
| WOS关键词 | READ ALIGNMENT ; OIL BODIES ; GENES ; IDENTIFICATION ; ANNOTATION ; RETROTRANSPOSONS ; CAROTENOIDS ; OLEOSINS ; SAPONINS ; DATABASE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000616721100015 |
| 出版者 | OXFORD UNIV PRESS INC |
| 资助机构 | Natural Science Foundation of Hunan Province [2019JJ50475] ; Key Scientific Research Projects of Hunan Education Department [18A448] ; Foundation of Hunan Double First-rate Discipline Construction Projects of Bioengineering and Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province ; National Science Foundation [81874334] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26718]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 2.Huaihua Univ, Coll Biol & Food Engn, Key Lab Res & Utilizat Ethnomed Plant Resources H, Huaihua 418000, Peoples R China 3.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Dao di Herbs, Beijing 100700, Peoples R China 4.Aarhus Univ, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark 5.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Hui,Liang, Juan,Tan, Qi,et al. Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis[J]. HORTICULTURE RESEARCH,2021,8(1). |
| APA | Huang, Hui.,Liang, Juan.,Tan, Qi.,Ou, Linfeng.,Li, Xiaolin.,...&Song, Songquan.(2021).Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis.HORTICULTURE RESEARCH,8(1). |
| MLA | Huang, Hui,et al."Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis".HORTICULTURE RESEARCH 8.1(2021). |
入库方式： OAI收割
来源：植物研究所
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