Identification of Transcription Factor Genes and Functional Characterization of PlMYB1 From Pueraria lobata
文献类型：期刊论文
| 作者 | Shen, Guoan; Wu, Ranran1; Xia, Yaying1,3; Pang, Yongzhen3 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2021 |
| 卷号 | 12 |
| 关键词 | Pueraria lobata transcriptome sequencing transcription factors MYB isoflavonoid biosynthesis |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2021.743518 |
| 文献子类 | Article |
| 英文摘要 | Kudzu, Pueraria lobata, is a traditional Chinese food and medicinal herb that has been commonly used since ancient times. Kudzu roots are rich sources of isoflavonoids, e.g., puerarin, with beneficial effects on human health. To gain global information on the isoflavonoid biosynthetic regulation network in kudzu, de novo transcriptome sequencings were performed using two genotypes of kudzu with and without puerarin accumulation in roots. RNAseq data showed that the genes of the isoflavonoid biosynthetic pathway were significantly represented in the upregulated genes in the kudzu with puerarin. To discover regulatory genes, 105, 112, and 143 genes encoding MYB, bHLH, and WD40 transcription regulators were identified and classified, respectively. Among them, three MYB, four bHLHs, and one WD40 gene were found to be highly identical to their orthologs involved in flavonoid biosynthesis in other plants. Notably, the expression profiles of PlMYB1, PlHLH3-4, and PlWD40-1 genes were closely correlated with isoflavonoid accumulation profiles in different tissues and cell cultures of kudzu. Over-expression of PlMYB1 in Arabidopsis thaliana significantly increased the accumulation of anthocyanins in leaves and proanthocyanidins in seeds, by activating AtDFR, AtANR, and AtANS genes. Our study provided valuable comparative transcriptome information for further identification of regulatory or structural genes involved in the isoflavonoid pathway in P. lobata, as well as for bioengineering of bioactive isoflavonoid compounds. |
| 学科主题 | Plant Sciences |
| 出版地 | LAUSANNE |
| WOS关键词 | TISSUE-SPECIFIC ANTHOCYANIN ; PROANTHOCYANIDIN BIOSYNTHESIS ; ECTOPIC EXPRESSION ; REGULATORY GENE ; PETUNIA ENCODES ; DOMAIN PROTEIN ; REPEAT PROTEIN ; GENOME-WIDE ; ARABIDOPSIS ; BHLH |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000710921300001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Science Foundation of China [31570306] ; Key Program of the National Natural Science Foundation of China [U20A2004] ; CAMS Innovation Fund for Medical Sciences (CIFMS) [2017-I2M-3-013] ; Agricultural Science and Technology Innovation Program [ASTIP-IAS10] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26730]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing, Peoples R China 2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing Bot Garden, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Guoan,Wu, Ranran,Xia, Yaying,et al. Identification of Transcription Factor Genes and Functional Characterization of PlMYB1 From Pueraria lobata[J]. FRONTIERS IN PLANT SCIENCE,2021,12. |
| APA | Shen, Guoan,Wu, Ranran,Xia, Yaying,&Pang, Yongzhen.(2021).Identification of Transcription Factor Genes and Functional Characterization of PlMYB1 From Pueraria lobata.FRONTIERS IN PLANT SCIENCE,12. |
| MLA | Shen, Guoan,et al."Identification of Transcription Factor Genes and Functional Characterization of PlMYB1 From Pueraria lobata".FRONTIERS IN PLANT SCIENCE 12(2021). |
入库方式： OAI收割
来源：植物研究所
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