LaPT2 Gene Encodes a Flavonoid Prenyltransferase in White Lupin
文献类型：期刊论文
| 作者 | Liu, Jinyue1,2,5; Xia, Yaying1,2,5; Jiang, Wenbo; Shen, Guoan4; Pang, Yongzhen |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2021 |
| 卷号 | 12 |
| 关键词 | white lupin flavonoids prenyltransferase LaPT2 kaempferol prenylation |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2021.673337 |
| 文献子类 | Article |
| 英文摘要 | Legume plants are rich in prenylated flavonoid compounds, which play an important role in plant defense and human health. In the present study, we identified a prenyltransferase (PT) gene, named LaPT2, in white lupin (Lupinus albus), which shows a high identity and close relationship with the other known PT genes involved in flavonoid prenylation in planta. The recombinant LaPT2 protein expressed in yeast cells exhibited a relatively strong activity toward several flavonols (e.g., kaempferol, quercetin, and myricetin) and a relatively weak activity toward flavanone (naringenin). In addition, the recombinant LaPT2 protein was also active toward several other types of flavonoids, including galangin, morin, 5-deoxyquercetin, 4'-O-methylkaempferol, taxifolin, and aromadendrin, with distinct enzymatic affinities. The LaPT2 gene was preferentially expressed in the roots, which is consistent with the presence of prenylated flavonoid kaempferol in the roots. Moreover, we found that the expression level of LaPT2 paralleled with those of LaF3H1 and LaFLS2 genes that were relatively higher in roots and lower in leaves, suggesting that they were essential for the accumulation of prenylated flavonoid kaempferol in roots. The deduced full-length LaPT2 protein and its signal peptide fused with a green fluorescent protein (GFP) are targeted to plastids in the Arabidopsis thaliana protoplast. Our study demonstrated that LaPT2 from white lupin is responsible for the biosynthesis of prenylated flavonoids, in particular flavonols, which could be utilized as phytoalexin for plant defense and bioactive flavonoid compounds for human health. |
| 学科主题 | Plant Sciences |
| 出版地 | LAUSANNE |
| WOS关键词 | SOPHORA-FLAVESCENS ; MOLECULAR CHARACTERIZATION ; PRENYLATED ISOFLAVONOIDS ; BIOSYNTHESIS ; GLYCEOLLIN ; FLAVANONE ; BIOLOGY ; CLONING ; ROOTS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000665741300001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Nature Science Foundation of China [U1906201] ; Basic Scientific Research Funding of CAAS [2019-YWF-YB-06] ; Central Publicinterest Scientific Institution Basal Research Fund [Y2020GH01-3] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26731]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China 3.Inst Med Plant Dev, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jinyue,Xia, Yaying,Jiang, Wenbo,et al. LaPT2 Gene Encodes a Flavonoid Prenyltransferase in White Lupin[J]. FRONTIERS IN PLANT SCIENCE,2021,12. |
| APA | Liu, Jinyue,Xia, Yaying,Jiang, Wenbo,Shen, Guoan,&Pang, Yongzhen.(2021).LaPT2 Gene Encodes a Flavonoid Prenyltransferase in White Lupin.FRONTIERS IN PLANT SCIENCE,12. |
| MLA | Liu, Jinyue,et al."LaPT2 Gene Encodes a Flavonoid Prenyltransferase in White Lupin".FRONTIERS IN PLANT SCIENCE 12(2021). |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。