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Flavan-3-ols in Vitis seeds: Their extraction and analysis by HPLC-ESI-MS/MS

文献类型：期刊论文

作者Wang, Junfang1,3; Zhang, Rui3; Jiang, Jinzhu3; Duan, Wei3; Fan, Peige3; Li, Shaohua3; Wang, Lijun3
刊名FOOD RESEARCH INTERNATIONAL
出版日期2021
卷号139
关键词Grape seeds Flavan-3-ols Extraction HPLC-ESI-MS/MS
ISSN号0963-9969
DOI10.1016/j.foodres.2020.109911
文献子类Article
英文摘要An orthogonal L(16)4(3) x 2(2) test design was applied to select the optimum conditions for extracting flavan-3-ols in grape seeds. Highest yield of flavan-3-ols was achieved with 80% methanol, a ratio [1:30 (g/mL)] of sample-to-solvent, sonication for 20 min, and extraction at 25 degrees C for 12 h in darkness. The optimized analytical method for HPLC separation was a multistep gradient elution using 1% formic acid (A) and acetonitrile containing 1% formic acid (B), at a flow rate of 0.6 mL/min in 36 min. Moreover, fourteen flavan-3-ols were separated and identified using HPLC-ESI-MS/MS, including four monomers ((+)-catechin, (-)-epicatechin, epigallocatechin gallate and epicatechin gallate) and ten oligomers (three dimers, four trimers, two tetramers and one pentamer). The optimized method was used to determine flavan-3-ols content and compositions among ten representative cultivars. The new wine grape - Beihong, had higher flavan-3-ols content and polymerization than classic wine grapes - Cabernet Sauvignon, Merlot, Semillon and Riesling.
学科主题Food Science & Technology
出版地AMSTERDAM
电子版国际标准刊号1873-7145
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000614713200014
出版者ELSEVIER
资助机构National Key R&D Program of China [2019YFD1000101] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA23080602] ; National Natural Science Foundation of China [31701887]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26736]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China
2.Shandong Acad Agr Sci, Key Lab Agroprod Proc Technol Shandong, Key Lab Novel Food Resources Proc, Minist Agr,Inst Agrofood Sci & Technol, Jinan 250100, Peoples R China
3.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Junfang,Zhang, Rui,Jiang, Jinzhu,et al. Flavan-3-ols in Vitis seeds: Their extraction and analysis by HPLC-ESI-MS/MS[J]. FOOD RESEARCH INTERNATIONAL,2021,139.
APA Wang, Junfang.,Zhang, Rui.,Jiang, Jinzhu.,Duan, Wei.,Fan, Peige.,...&Wang, Lijun.(2021).Flavan-3-ols in Vitis seeds: Their extraction and analysis by HPLC-ESI-MS/MS.FOOD RESEARCH INTERNATIONAL,139.
MLA Wang, Junfang,et al."Flavan-3-ols in Vitis seeds: Their extraction and analysis by HPLC-ESI-MS/MS".FOOD RESEARCH INTERNATIONAL 139(2021).

入库方式： OAI收割

来源：植物研究所

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