Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice
文献类型：期刊论文
| 作者 | Xing, Mengyang2; Chen, Yong; Li, Boqiang; Tian, Shiping2
|
| 刊名 | FOOD CHEMISTRY
 |
| 出版日期 | 2021 |
| 卷号 | 348 |
| 关键词 | Patulin Degradation Short-chain dehydrogenase reductase Apple juice |
| ISSN号 | 0308-8146 |
| DOI | 10.1016/j.foodchem.2021.129046 |
| 文献子类 | Article |
| 英文摘要 | Biodegradation based on microbial enzymes is considered to be one of the promising ways for controlling patulin contamination. However, few patulin degrading enzymes have been isolated and characterized until now. Here, a short-chain dehydrogenase/reductase (SDR) gene, CgSDR, was cloned from a yeast strain Candida guilliermondii, and expressed in Escherichia coli. The expression of CgSDR conferred a strong patulin tolerance and degradation ability to E. coli, and purified CgSDR could transform patulin into E-ascladiol in vitro with NADPH as a coenzyme. Moreover, addition of CgSDR at 150 mu g/mL could reduce 80% of patulin in apple juice and the biodegradation process did not affect the quality of the apple juice. A molecular docking analysis and site-directed mutagenesis indicated that CgSDR might interact with patulin via VAL188 as an active binding sites. The findings provide new insights for developing enzymic formulations for mycotoxin detoxification in fruit derived products. |
| 学科主题 | Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1873-7072 |
| WOS关键词 | MYCOTOXIN PATULIN ; SUPERFAMILY ; SDR ; CLASSIFICATION ; DEGRADATION ; PH |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000623049400012 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | National Natural Science Foundation of China [31930086, 31722043] ; National Key R&D Program of China [2016YFD0400902] ; Youth Innovation Promotion Association, CAS [Y201919] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26764]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xing, Mengyang,Chen, Yong,Li, Boqiang,et al. Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice[J]. FOOD CHEMISTRY,2021,348. |
| APA | Xing, Mengyang,Chen, Yong,Li, Boqiang,&Tian, Shiping.(2021).Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice.FOOD CHEMISTRY,348. |
| MLA | Xing, Mengyang,et al."Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice".FOOD CHEMISTRY 348(2021). |
入库方式： OAI收割
来源：植物研究所
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