A Carvacrol-Rich Essential Oil Extracted From Oregano (Origanum vulgare Hot & Spicy) Exerts Potent Antibacterial Effects Against Staphylococcus aureus
文献类型：期刊论文
| 作者 | Hao, Yuanpeng1,2; Li, Jingyi1; Shi, Lei1
|
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2021 |
| 卷号 | 12 |
| 关键词 | carvacrol cell membrane oregano essential oil proteomics Staphylococcus aureus |
| DOI | 10.3389/fmicb.2021.741861 |
| 文献子类 | Article |
| 英文摘要 | Oregano essential oil (OEO), as a natural antimicrobial, has gained increased interest from food researchers and manufacturers. However, a few studies have investigated its possible antibacterial effects against Staphylococcus aureus using the proteomic tool. The present study aimed to explore the antibacterial effect and mechanism of a carvacrol-rich OEO extracted from Origanum vulgare Hot & Spicy on the inactivation of S. aureus. The gas chromatography-mass spectrometry analysis of the OEO allowed the detection of 27 compounds; the major constituent was carvacrol (84.38% of total compounds). The average diameter of the inhibitory zone (DIZ) value was 29.10 mm, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of OEO against S. aureus were 0.125 and 0.25 mg/mL, respectively. The growth curve assay indicated that the OEO prolonged the lag phase of S. aureus. The decrease in cell viability, changes in the integrity of cell membrane, and abnormal cell morphology further reflected the cell damage of S. aureus caused by the OEO. In addition, a label-free proteomic analysis was applied to analyze the regulatory networks of S. aureus in response to 1/2 MIC OEO-treatment stress. Of the 56 differentially expressed proteins (DEPs) identified, 26 were significantly upregulated and 30 downregulated. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that the DEPs were mainly involved in pathways of ribosomes; valine, leucine, and isoleucine biosynthesis; and phenylalanine, tyrosine, and tryptophan biosynthesis, which suggested that the growth inhibition of S. aureus might be due to the disordered effect of the OEO on protein synthesis and amino acid metabolism. These findings deepened our understanding regarding S. aureus survival and metabolism responses to the OEO treatment and suggested that the carvacrol-rich OEO could be used in food production environments to effectively control S. aureus. |
| 学科主题 | Microbiology |
| 出版地 | LAUSANNE |
| 电子版国际标准刊号 | 1664-302X |
| WOS关键词 | ANTIMICROBIAL ACTIVITY ; THYMOL ; L. |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000720323500001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26794]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Yuanpeng,Li, Jingyi,Shi, Lei. A Carvacrol-Rich Essential Oil Extracted From Oregano (Origanum vulgare Hot & Spicy) Exerts Potent Antibacterial Effects Against Staphylococcus aureus[J]. FRONTIERS IN MICROBIOLOGY,2021,12. |
| APA | Hao, Yuanpeng,Li, Jingyi,&Shi, Lei.(2021).A Carvacrol-Rich Essential Oil Extracted From Oregano (Origanum vulgare Hot & Spicy) Exerts Potent Antibacterial Effects Against Staphylococcus aureus.FRONTIERS IN MICROBIOLOGY,12. |
| MLA | Hao, Yuanpeng,et al."A Carvacrol-Rich Essential Oil Extracted From Oregano (Origanum vulgare Hot & Spicy) Exerts Potent Antibacterial Effects Against Staphylococcus aureus".FRONTIERS IN MICROBIOLOGY 12(2021). |
入库方式： OAI收割
来源：植物研究所
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