A new, unquenched intermediate of LHCII
文献类型：期刊论文
| 作者 | Li, Fei; Liu, Cheng; Streckaite, Simona; Yang, Chunhong; Xu, Pengqi3; Llansola-Portoles, Manuel J.; Ilioaia, Cristian; Pascal, Andrew A.; Croce, Roberta3; Robert, Bruno1 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2021 |
| 卷号 | 296 |
| DOI | 10.1016/j.jbc.2021.100322 |
| 文献子类 | Article |
| 英文摘要 | When plants are exposed to high-light conditions, the potentially harmful excess energy is dissipated as heat, a process called non-photochemical quenching. Efficient energy dissipation can also be induced in the major light-harvesting complex of photosystem II (LHCII) in vitro, by altering the structure and interactions of several bound cofactors. In both cases, the extent of quenching has been correlated with conformational changes (twisting) affecting two bound carotenoids, neoxanthin, and one of the two luteins (in site L1). This lutein is directly involved in the quenching process, whereas neoxanthin senses the overall change in state without playing a direct role in energy dissipation. Here we describe the isolation of an intermediate state of LHCII, using the detergent ndodecyl-alpha-D-maltoside, which exhibits the twisting of neoxanthin (along with changes in chlorophyll-protein interactions), in the absence of the L1 change or corresponding quenching. We demonstrate that neoxanthin is actually a reporter of the LHCII environment-probably reflecting a large-scale conformational change in the protein-whereas the appearance of excitation energy quenching is concomitant with the configuration change of the L1 carotenoid only, reflecting changes on a smaller scale. This unquenched LHCII intermediate, described here for the first time, provides for a deeper understanding of the molecular mechanism of quenching. |
| 学科主题 | Biochemistry & Molecular Biology |
| 出版地 | AMSTERDAM |
| 电子版国际标准刊号 | 1083-351X |
| WOS关键词 | LIGHT-HARVESTING-COMPLEX ; CIS-TRANS ISOMERS ; RESONANCE RAMAN ; PHOTOSYSTEM-II ; BETA-CAROTENE ; CHLOROPHYLL FLUORESCENCE ; PHOTOSYNTHETIC BACTERIA ; ELECTRONIC ABSORPTION ; PIGMENT STRUCTURE ; REACTION CENTERS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000672866400296 |
| 出版者 | ELSEVIER |
| 资助机构 | French Infrastructure for Integrated Structural Biology (FRISBI) [ANR-10-INBS-05] ; Infrastructures en Biologie Sante et Agronomie (IBiSA), Sino-French Cai Yuanpei Programme [Ou Liu Jin [2016] 6313] ; National Natural Science Foundation of China [31570236] ; De Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) [86510013] ; China Scholarship Council (CSC) [201604490129] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26806]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Vrije Univ Amsterdam, Fac Sci, Dept Phys & Astron, Amsterdam, Netherlands 3.Univ Paris Saclay, CEA, CNRS, Inst Integrat Biol Cell I2BC, Gif Sur Yvette, France |
| 推荐引用方式 GB/T 7714 | Li, Fei,Liu, Cheng,Streckaite, Simona,et al. A new, unquenched intermediate of LHCII[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2021,296. |
| APA | Li, Fei.,Liu, Cheng.,Streckaite, Simona.,Yang, Chunhong.,Xu, Pengqi.,...&Robert, Bruno.(2021).A new, unquenched intermediate of LHCII.JOURNAL OF BIOLOGICAL CHEMISTRY,296. |
| MLA | Li, Fei,et al."A new, unquenched intermediate of LHCII".JOURNAL OF BIOLOGICAL CHEMISTRY 296(2021). |
入库方式： OAI收割
来源：植物研究所
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