Identification of Specific Glycosyltransferases Involved in Flavonol Glucoside Biosynthesis in Ginseng Using Integrative Metabolite Profiles, DIA Proteomics, and Phylogenetic Analysis
文献类型：期刊论文
| 作者 | Yin, Qinggang2,4; Han, Xiaoyan; Chen, Jingwang1; Han, Zongxian2; Shen, Liang2,3; Sun, Wei2; Chen, Shilin2 |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2021 |
| 卷号 | 69期号:5页码:1714-1726 |
| 关键词 | flavonol glycoside proteomics glucosyltransferase galacosyltransferase Panax ginseng |
| ISSN号 | 0021-8561 |
| DOI | 10.1021/acs.jafc.0c06989 |
| 文献子类 | Article |
| 英文摘要 | Ginseng contains a variety of flavonol glycosides that possess diverse biological activities; however, scant information of flavonoid glycosylation was reported in ginseng. We found that panasenoside and kaempferol 3-O-glucoside were commonly accumulated along with cultivation years in leaves. In order to explore the procedure of flavonol glycosylation in ginseng, 50 UDP-glycosyltransferases (UGTs) were screened out using differentiated data-independent acquisition (DIA) proteomics and phylogenetic analysis. UGT92A10 and UGT94Q4 were found contributing to the formation of kaempferol 3-O-glucoside. UGT73A18, UGT74T4, and UGT75W1 could catalyze galactosylation of kaempferol 3-O-glucoside. Ser278, Trp335, Gln338, and Val339 were found forming hydrogen bonds with UDP-galactose in UGT75W1 by docking. MeJA induced transcripts of UGT73A18 and UGT74T4 by over fourfold, consistent with the decrease of kaempferol 3-O-glucoside, which indicated that these genes may be related to resisting adversity stress in ginseng. These results highlight the significance of integrative metabolite profiles, proteomics, and phylogenetic analysis for exploring flavonol glycosylation in ginseng. |
| 学科主题 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| 出版地 | WASHINGTON |
| 电子版国际标准刊号 | 1520-5118 |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000619637900025 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Key R&D Program of China [2019YFC1711100] ; Fundamental Research Funds for the Central public welfare research institutes [ZZ13-YQ-097] ; China Postdoctoral Science Foundation [2018 M631705] ; National Science and Technology Major Project Creation of Major New Drugs [2019ZX09201005-006-004, 2019ZX09731-002] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26365]  |
| 专题 | 植物园 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 2.Chinese Acad Agr Sci, Inst Food Sci & Technol, Key Lab Agroprod Proc, Minist Agr, Beijing 100193, Peoples R China 3.China Acad Chinese Med Sci, Inst Chinese Mat Med, Key Lab Beijing Identificat & Safety Evaluat Chin, Beijing 100700, Peoples R China 4.Beijing Acad Sci & Technol, Beijing Museum Nat Hist, Beijing 100050, Peoples R China 5.China Acad Chinese Med Sci, Artemisinin Res Ctr, Beijing 100700, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Qinggang,Han, Xiaoyan,Chen, Jingwang,et al. Identification of Specific Glycosyltransferases Involved in Flavonol Glucoside Biosynthesis in Ginseng Using Integrative Metabolite Profiles, DIA Proteomics, and Phylogenetic Analysis[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2021,69(5):1714-1726. |
| APA | Yin, Qinggang.,Han, Xiaoyan.,Chen, Jingwang.,Han, Zongxian.,Shen, Liang.,...&Chen, Shilin.(2021).Identification of Specific Glycosyltransferases Involved in Flavonol Glucoside Biosynthesis in Ginseng Using Integrative Metabolite Profiles, DIA Proteomics, and Phylogenetic Analysis.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,69(5),1714-1726. |
| MLA | Yin, Qinggang,et al."Identification of Specific Glycosyltransferases Involved in Flavonol Glucoside Biosynthesis in Ginseng Using Integrative Metabolite Profiles, DIA Proteomics, and Phylogenetic Analysis".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 69.5(2021):1714-1726. |
入库方式： OAI收割
来源：植物研究所
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