N-glucosyltransferase GbNGT1 from ginkgo complements the auxin metabolic pathway
文献类型：期刊论文
| 作者 | Yin, Qinggang4; Zhang, Jing; Wang, Shuhui; Cheng, Jintang; Gao, Han; Guo, Cong; Ma, Lianbao5; Sun, Limin1; Han, Xiaoyan3; Chen, Shilin |
| 刊名 | HORTICULTURE RESEARCH
 |
| 出版日期 | 2021 |
| 卷号 | 8期号:1 |
| ISSN号 | 2662-6810 |
| DOI | 10.1038/s41438-021-00658-0 |
| 文献子类 | Article |
| 英文摘要 | As auxins are among the most important phytohormones, the regulation of auxin homeostasis is complex. Generally, auxin conjugates, especially IAA glucosides, are predominant at high auxin levels. Previous research on terminal glucosylation focused mainly on the O-position, while IAA-N-glucoside and IAA-Asp-N-glucoside have been neglected since their discovery in 2001. In our study, IAA-Asp-N-glucoside was found to be specifically abundant (as high as 4.13 mg/g) in the seeds of 58 ginkgo cultivars. Furthermore, a novel N-glucosyltransferase, termed GbNGT1, was identified via differential transcriptome analysis and in vitro enzymatic testing. It was found that GbNGT1 could catalyze IAA-Asp and IAA to form their corresponding N-glucosides. The enzyme was demonstrated to possess a specific catalytic capacity toward the N-position of the IAA-amino acid or IAA from 52 substrates. Docking and site-directed mutagenesis of this enzyme confirmed that the E15G mutant could almost completely abolish its N-glucosylation ability toward IAA-Asp and IAA in vitro and in vivo. The IAA modification of GbNGT1 and GbGH3.5 was verified by transient expression assay in Nicotiana benthamiana. The effect of GbNGT1 on IAA distribution promotes root growth in Arabidopsis thaliana. |
| 学科主题 | Plant Sciences ; Genetics & Heredity ; Horticulture |
| 出版地 | CARY |
| 电子版国际标准刊号 | 2052-7276 |
| WOS关键词 | BIOCHEMICAL-CHARACTERIZATION ; GLYCOSYLTRANSFERASE ; ARABIDOPSIS ; FAMILY ; ACID ; IDENTIFICATION ; GLYCOSYLATION ; BIOSYNTHESIS ; DERIVATIVES ; EXPRESSION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000713155100010 |
| 出版者 | OXFORD UNIV PRESS INC |
| 资助机构 | Scientific and technological innovation project of China Academy of Chinese Medical Sciences (CACMS Innovation Fund) [C12021A04117] ; National Key R&D Program of China [2019YFC1711100] ; Beijing Natural Science Foundation of China [7192138] ; Fundamental Research Funds for the Central Public Welfare Research Institute of China [ZZ13-YQ-097] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26543]  |
| 专题 | 植物园 |
| 作者单位 | 1.Inst Ginkgo, Pizhou 221300, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 3.Shandong Agr Univ, Coll Forestry, State Forestry & Grassland Adm, Key Lab Silviculture Downstream Areas Yellow Rive, Tai An 271000, Shandong, Peoples R China 4.China Acad Chinese Med Sci, Inst Chinese Mat Med, Key Lab Beijing Identificat & Safety Evaluat Chin, Beijing 100700, Peoples R China 5.China Acad Chinese Med Sci, Artemisinin Res Ctr, Beijing 100700, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Qinggang,Zhang, Jing,Wang, Shuhui,et al. N-glucosyltransferase GbNGT1 from ginkgo complements the auxin metabolic pathway[J]. HORTICULTURE RESEARCH,2021,8(1). |
| APA | Yin, Qinggang.,Zhang, Jing.,Wang, Shuhui.,Cheng, Jintang.,Gao, Han.,...&Liu, An.(2021).N-glucosyltransferase GbNGT1 from ginkgo complements the auxin metabolic pathway.HORTICULTURE RESEARCH,8(1). |
| MLA | Yin, Qinggang,et al."N-glucosyltransferase GbNGT1 from ginkgo complements the auxin metabolic pathway".HORTICULTURE RESEARCH 8.1(2021). |
入库方式： OAI收割
来源：植物研究所
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