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Preparation and structural regulation of macroporous agarose microspheres for highly efficient adsorption of giant biomolecules

文献类型：期刊论文

作者Zhao, Lan4; Huang, Lan1,4; Huang, Yongdong4; Zhu, Kai4; Che, Xiangjing2,4; Du, Yuxiang1,4; Gao, Jingwei1,4; Hao, Dongxia4; Zhang, Rongyue3; Wang, Qibao1
刊名COLLOID AND POLYMER SCIENCE
出版日期2022-06-01
卷号300期号:6页码:691-705
ISSN号0303-402X
关键词Macroporous agarose microspheres Micellar swelling method Pore-forming Chromatographic media Adsorption
DOI10.1007/s00396-022-04968-0
英文摘要Macroporous agarose microspheres for bioseparation of giant biomolecules were prepared by a surfactant micellar swelling method, and the effects of preparation conditions on both particle size and its distribution and pore structure were systematically studied. Under the optimal condition with a surfactant amount of 20% (v/v), Triton X-100 to sodium dodecyl sulfate ratio (SDS) of 5:1 (w/w), an oil-absorbing-swelling time of 2.5 h, and a stirring speed of 250 rpm, macroporous agarose microspheres were successfully prepared, characterized by inverse size exclusion chromatography. Distribution coefficient (K-d) of dextran probes for agarose microspheres after being cross-linked was almost the same as that for agarose microspheres before being cross-linked. Inverse size exclusion chromatography results showed that macroporous agarose microspheres had a pore size distribution of more than 40 nm or even close to 60 nm, while that of conventional microspheres was no more than 40 nm. Macroporous agarose microspheres functionalized with diethylaminoethyl groups had a higher adsorption capacity and faster adsorption rate for hepatitis B surface antigen (HBsAg) compared with both commercial DEAE-agarose chromatographic media and conventional DEAE-agarose microspheres which were both based on agarose microspheres prepared using a traditional method. Confocal laser scanning microscopy results showed that macroporous agarose microspheres had a good binding ability to HBsAg, and this antigen completely entered the inside of the microspheres. Compared with conventional agarose chromatographic media, the macroporous agarose media combined macromolecular proteins more quickly and fully, having wider applications in the field of purification of giant biomolecules.
WOS关键词SUPERPOROUS AGAROSE ; CHROMATOGRAPHY ; PURIFICATION ; MONOLITHS ; BEADS
资助项目National Key Research and Development Program of China[2021YFC2103402] ; National Key Research and Development Program of China[2021YFC2102802] ; National Key Research and Development Program of China[2018YFC0311101] ; Beijing Natural Science Foundation[L192017] ; National Natural Science Foundation of China[21821005] ; National Natural Science Foundation of China[22078334] ; National Natural Science Foundation of China[21902160] ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee[ZZB2019001]
WOS研究方向Chemistry ; Polymer Science
语种英语
出版者SPRINGER
WOS记录号WOS:000792975700002
资助机构National Key Research and Development Program of China ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee
源URL[http://ir.ipe.ac.cn/handle/122111/53292]  
专题中国科学院过程工程研究所
通讯作者Huang, Yongdong; Ma, Guanghui
作者单位1.China Univ Min & Technol, Sch Chem & Environm Engn, Beijing 100083, Peoples R China
2.Yanshan Univ, Coll Environm & Chem Engn, Qinhuangdao 066000, Hebei, Peoples R China
3.Beijing Inst Petrochem Technol, Dept Appl Chem, Beijing 102617, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Lan,Huang, Lan,Huang, Yongdong,et al. Preparation and structural regulation of macroporous agarose microspheres for highly efficient adsorption of giant biomolecules[J]. COLLOID AND POLYMER SCIENCE,2022,300(6):691-705.
APA Zhao, Lan.,Huang, Lan.,Huang, Yongdong.,Zhu, Kai.,Che, Xiangjing.,...&Ma, Guanghui.(2022).Preparation and structural regulation of macroporous agarose microspheres for highly efficient adsorption of giant biomolecules.COLLOID AND POLYMER SCIENCE,300(6),691-705.
MLA Zhao, Lan,et al."Preparation and structural regulation of macroporous agarose microspheres for highly efficient adsorption of giant biomolecules".COLLOID AND POLYMER SCIENCE 300.6(2022):691-705.

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来源：过程工程研究所

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