A Short Peptide of Autotransporter Ata Is a Promising Protective Antigen for Vaccination Against Acinetobacter baumannii
文献类型：期刊论文
| 作者 | Sun, Peng2,3; Li, Xin1,2; Pan, Chao2; Liu, Zhicheng2; Wu, Jun2; Wang, Hengliang2,3; Zhu, Li2 |
| 刊名 | FRONTIERS IN IMMUNOLOGY
 |
| 出版日期 | 2022-04-13 |
| 卷号 | 13页码:12 |
| 关键词 | Acinetobacter baumannii acinetobacter trimeric autotransporter adhesin (Ata) CpG CTB subunit vaccine |
| ISSN号 | 1664-3224 |
| DOI | 10.3389/fimmu.2022.884555 |
| 英文摘要 | With the emergence of multidrug-resistant strains, Acinetobacter baumannii infection is becoming a thorny health problem in hospitals. However, there are no licensed vaccines against A. baumannii. Acinetobacter trimeric autotransporter (Ata) is an important known virulence factor located on the outer membrane of bacteria. Herein, we carried out a series of experiments to test the immunogenicity of a short C-terminal extracellular region of Ata (Ata(alpha), only containing 39 amino acids) in a murine model. The short peptide Ata(alpha) was fused with the cholera toxin B subunit (CTB), which has been reported to have immunoadjuvant activity. The fusion protein showed no inflammation and organ damages, and have the ability to elicit both Th1 and Th2 immune responses in mice. The bactericidal activities against A. baumannii and prophylactic effects of the fusion protein were further evidenced by a significant reduction in the bacterial load in the organs and blood. In addition, the candidate vaccine could provide broad protection against lethal challenges with a variety of A. baumannii strains. Moreover, when CpG was added on the basis of aluminum adjuvant, the immune response, especially cellular immunity, could be further strengthened. Overall, these results revealed that the Ata(alpha) is a promising vaccine target against A. baumannii infection. |
| WOS关键词 | TOXIN B-SUBUNIT ; LINKED GLYCOSYLATION SYSTEM ; DENDRITIC CELL VACCINATION ; CHOLERA-TOXIN ; TRIMERIC AUTOTRANSPORTER ; CPG OLIGODEOXYNUCLEOTIDE ; HELICOBACTER-PYLORI ; IMMUNE-RESPONSES ; INFECTION ; INDUCTION |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000792571500001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/53309]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Wu, Jun; Wang, Hengliang; Zhu, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 2.Beijing Inst Biotechnol, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China 3.Tsinghua Univ, Sch Med, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Peng,Li, Xin,Pan, Chao,et al. A Short Peptide of Autotransporter Ata Is a Promising Protective Antigen for Vaccination Against Acinetobacter baumannii[J]. FRONTIERS IN IMMUNOLOGY,2022,13:12. |
| APA | Sun, Peng.,Li, Xin.,Pan, Chao.,Liu, Zhicheng.,Wu, Jun.,...&Zhu, Li.(2022).A Short Peptide of Autotransporter Ata Is a Promising Protective Antigen for Vaccination Against Acinetobacter baumannii.FRONTIERS IN IMMUNOLOGY,13,12. |
| MLA | Sun, Peng,et al."A Short Peptide of Autotransporter Ata Is a Promising Protective Antigen for Vaccination Against Acinetobacter baumannii".FRONTIERS IN IMMUNOLOGY 13(2022):12. |
入库方式： OAI收割
来源：过程工程研究所
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