Fast and high-resolution fractionation of positional isomers of a PEGylated protein using membrane chromatography
文献类型：期刊论文
| 作者 | Chen, Guoqiang1; Butani, Nikhila2; Ghosh, Raja2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2022-07-01 |
| 卷号 | 1203页码:8 |
| 关键词 | PEGylated protein Membrane chromatography Z(2)LFMC Positional isomers Cation-exchange Purification |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2022.123292 |
| 英文摘要 | The fractionation of positional isomers of a PEGylated protein is quite challenging as these have similar mo-lecular weight, and only very slightly different surface charge. In this study, cation exchange z2 laterally-fed membrane chromatography (z2LFMC), which has been shown to be suitable for high-speed, high-resolution protein purification, was used to fractionate positional isomers of mono-PEGylated lysozyme. The performance of the z2LFMC device was compared with a commercial preparative cation exchange column having the same volume and ligand. PEGylated lysozyme purification experiments showed that while the positional isomers of mono-PEGylated lysozyme could not be satisfactorily resolved using the preparative commercial cation exchange column, almost baseline resolution of these could be achieved using the z2LFMC device. Moreover, the z2LFMC device-based process was faster by an order of magnitude. The results discussed in this paper demonstrate that z2LFMC is a superior alternative to column-based chromatography for challenging protein separations, such as the one discussed here, both in terms of speed and resolution. |
| WOS关键词 | PREPARATIVE SEPARATION ; PURIFICATION ; PERFORMANCE ; HIRUDIN |
| 资助项目 | Natural Science and Engineering Research Council (NSERC) of Canada |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000804671600001 |
| 出版者 | ELSEVIER |
| 资助机构 | Natural Science and Engineering Research Council (NSERC) of Canada |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/53639]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Ghosh, Raja |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China 2.McMaster Univ, Dept Chem Engn, 1280 Main St West, Hamilton, ON L8S 4L7, Canada |
| 推荐引用方式 GB/T 7714 | Chen, Guoqiang,Butani, Nikhila,Ghosh, Raja. Fast and high-resolution fractionation of positional isomers of a PEGylated protein using membrane chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2022,1203:8. |
| APA | Chen, Guoqiang,Butani, Nikhila,&Ghosh, Raja.(2022).Fast and high-resolution fractionation of positional isomers of a PEGylated protein using membrane chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1203,8. |
| MLA | Chen, Guoqiang,et al."Fast and high-resolution fractionation of positional isomers of a PEGylated protein using membrane chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1203(2022):8. |
入库方式： OAI收割
来源：过程工程研究所
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