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Ribosome-binding Sequences (RBS) Engineering of Key Genes in Escherichia coli for High Production of Fatty Alcohols

文献类型：期刊论文

作者Chen, Ruonan1,3; Liu, Yilan1; Zhong, Wei1; Hao, Xuemi1; Mu, Tingzhen1; Yang, Maohua1; Xing, Jianmin1,2,4
刊名BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
出版日期2022-05-31
页码9
ISSN号1226-8372
关键词fatty alcohol fatty acid ribosome-binding sequence calculator acyl-ACP reductase synthetic biology
DOI10.1007/s12257-021-0354-0
英文摘要Fatty alcohols are widely used in the fields of food, pharmaceutical and renewable energy and so on, thus biosynthesis of fatty alcohols has attracted wide attention in recent years. However, the concentration of fatty alcohols is still at a low level. In this paper, we constructed an Escherichia coli strain with high yield of fatty alcohols through the combinatorial regulation of three key genes of fabH, fabZ, and far. Firstly, we designed new ribosome-binding sequences (RBS) of fabH and fabZ with 10-fold strengths compared to the original ones by RBS calculator, separately. Secondly, we engineered the starting strain MGL1 by replacing the original RBS with designed ones, which resulted in MGLHS (fabH-RBS1), MGLZS (fabZ-RBS2), and MGLHZS (fabH-RBS1/fabZ-RBS2), respectively. Thirdly, the plasmid pL1 harboring far gene was transferred into above strains to test fatty alcohols production under the induction of different isopropyl-beta-D-thiogalactoside concentrations. The results showed that the concentration of fatty alcohols in strains MGLHS-far, MGLZS-far and MGLHZS-far reached 2.75 g/L, 2.54 g/L and 3.82 g/L, respectively, which increased 35%, 25% and 88% than that of the starting strain MGL1-far. The fatty alcohols reached 9.35 g/L in fed batch fermentation with MGLHZS-far, of which the main components were two saturated fatty alcohols (C14:0 and C16:0) and two unsaturated fatty alcohols (C16:1 and C18:1). The results demonstrated that combinatorial regulation of three key genes of fabH, fabZ, and far in E. coli can achieve high-yield production of fatty alcohols.
WOS关键词THERMODYNAMIC PARAMETERS ; TRANSLATIONAL EFFICIENCY ; MICROBIAL-PRODUCTION ; ACYL-COA ; REDUCTASE ; PROTEIN ; FUELS ; RNA ; SYNTHASE ; BIOMASS
资助项目National Key Rsearch & Development Program of China[2019YFB1503805] ; National Key Rsearch & Development Program of China[2019YFC1906602]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
出版者KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
WOS记录号WOS:000803857800002
资助机构National Key Rsearch & Development Program of China
源URL[http://ir.ipe.ac.cn/handle/122111/53669]  
专题中国科学院过程工程研究所
通讯作者Liu, Yilan; Yang, Maohua; Xing, Jianmin
作者单位1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
2.Guangdong Lab, Chem & Chem Engn, Shantou 515031, Peoples R China
3.Beijing Univ Chem Technol, Beijing 100029, Peoples R China
4.Univ Chinese Acad Sci, Coll Chem Engn, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Ruonan,Liu, Yilan,Zhong, Wei,et al. Ribosome-binding Sequences (RBS) Engineering of Key Genes in Escherichia coli for High Production of Fatty Alcohols[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2022:9.
APA Chen, Ruonan.,Liu, Yilan.,Zhong, Wei.,Hao, Xuemi.,Mu, Tingzhen.,...&Xing, Jianmin.(2022).Ribosome-binding Sequences (RBS) Engineering of Key Genes in Escherichia coli for High Production of Fatty Alcohols.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,9.
MLA Chen, Ruonan,et al."Ribosome-binding Sequences (RBS) Engineering of Key Genes in Escherichia coli for High Production of Fatty Alcohols".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING (2022):9.

入库方式： OAI收割

来源：过程工程研究所

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