Extraction and characterization of bovine collagen Type V and its effects on cell behaviors
文献类型：期刊论文
| 作者 | Xu, Jun2,3; Luo, Xi1; Zhang, Yang1; Gao, Jianping1; Huang, Ching-Cheng3,4; Bai, Xinpeng2,3; Zhang, Guifeng1 |
| 刊名 | REGENERATIVE BIOMATERIALS
 |
| 出版日期 | 2022-04-08 |
| 卷号 | 9页码:11 |
| ISSN号 | 2056-3418 |
| 关键词 | collagen Type V bovine cornea fiber diameter cell migration |
| DOI | 10.1093/rb/rbac028 |
| 英文摘要 | Collagen Type V (Col. V) plays an essential role in cell behaviors and has attracted increasing attention in recent years. High-purity Col. V is needed for evaluating its biological properties. In this research, the enzymatic hydrolysis process was combined with ultrafiltration to purify Col. V from the bovine cornea. The purity of Col. V was determined to be above 90% by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance liquid chromatography methods. The effect of Col. V on cell behaviors was evaluated. The circular dichroism spectroscopy results demonstrated that the extracted Col. V exhibited a complete triple helix structure. SDS-PAGE suggested that the molecular weight of Col. V was 440 kDa. The self-assembly experiment revealed that the proportion of Col. V in the collagen mixture can affect the Col. I fiber diameter. The cell culture results implied that Col. V can inhibit fibroblasts (L929) proliferation. The L929 showed maximum mobility when the addition of Col. V was 30%. Thus, Col. V has the effect of inhibiting L929 proliferation and promoting migration. The high-purity Col. V provides useful information for further understanding its biological implications. |
| WOS关键词 | SKIN ; SPECTROSCOPY ; MEMBRANE ; ADHESION ; SCALES |
| 资助项目 | National Key Technology R&D Programs of China[2021YFC2400800] ; Science and Technology Program of Guangzhou, China[201803010086] ; Open Funding Project of the State Key Laboratory of Biochemical Engineering[2021KF-04] ; Independent Research Project of the State Key Laboratory of Biochemical Engineering[2021ZZ-03] |
| WOS研究方向 | Materials Science |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000811792200001 |
| 资助机构 | National Key Technology R&D Programs of China ; Science and Technology Program of Guangzhou, China ; Open Funding Project of the State Key Laboratory of Biochemical Engineering ; Independent Research Project of the State Key Laboratory of Biochemical Engineering |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/53933]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Huang, Ching-Cheng; Bai, Xinpeng; Zhang, Guifeng |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Hainan Univ, Coll Food Sci & Engn, Engn Res Ctr Utilizat Trop Polysaccharide Resourc, Minist Educ, Haikou 570228, Hainan, Peoples R China 3.PARSD Biomed Mat Res Ctr Changzhou, Changzhou 213176, Peoples R China 4.Ming Chuan Univ, Dept Biomed Engn, Taipei 32033, Taiwan |
| 推荐引用方式 GB/T 7714 | Xu, Jun,Luo, Xi,Zhang, Yang,et al. Extraction and characterization of bovine collagen Type V and its effects on cell behaviors[J]. REGENERATIVE BIOMATERIALS,2022,9:11. |
| APA | Xu, Jun.,Luo, Xi.,Zhang, Yang.,Gao, Jianping.,Huang, Ching-Cheng.,...&Zhang, Guifeng.(2022).Extraction and characterization of bovine collagen Type V and its effects on cell behaviors.REGENERATIVE BIOMATERIALS,9,11. |
| MLA | Xu, Jun,et al."Extraction and characterization of bovine collagen Type V and its effects on cell behaviors".REGENERATIVE BIOMATERIALS 9(2022):11. |
入库方式： OAI收割
来源：过程工程研究所
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