Dynamic process enhancement on chitosan/gelatin/nano-hydroxyapatite-bone derived multilayer scaffold for osteochondral tissue repair
文献类型：期刊论文
| 作者 | Hu, Xueyan2; Zheng, Shuangshuang1; Zhang, Rui2; Wang, Yiwei4; Jiao, Zeren5; Li, Wenfang2,3; Nie, Yi1,6; Liu, Tianqing2; Song, Kedong2 |
| 刊名 | BIOMATERIALS ADVANCES
 |
| 出版日期 | 2022-02-01 |
| 卷号 | 133页码:17 |
| 关键词 | Chitosan gelatin nano-hydroxyapatite multilayer scaffold Bone-derived scaffold Dynamic environment Osteochondral tissue regeneration |
| DOI | 10.1016/j.msec.2022.112662 |
| 英文摘要 | Accurate repair of osteochondral defects is a great challenge due to the complex structure of osteochondral defects. The current study aims to prepare a biomimetic osteochondral gradient scaffold based on chitosan, gelatin and nanohydroxyapatite and bone-derived materials for repair cartilage defects. Hereon, the chitosan/gelatin/nanohydroxyapatite multilayer scaffold with complex hierarchical structure using iterative hierarchical method is prepared to simulate the structure of natural cartilage. And porcine femur in distal metaphysis was treated by chemical decellularization, deproteinization, decalcification, and degreasing to obtain the bone-derived scaffold simulating subchondral bone layer. We also used a spinner bioreactor for the in vitro modeling of the microenvironment which can promote adipose mesenchymal stem cell (ADSCs) recruitment into the chitosan/gelatin/nano-hydroxyapatitebone derived multilayer scaffold under physiological flow conditions. Biological experiments have shown that osteochondral layered materials can induce ADSCs to differentiate into chondrocytes and osteoblasts and exhibit chondrogenic and osteogenic phenotypes similar to natural tissues, respectively. Furthermore, the expression both of the chondrogenic gene (Col II, ACAN, and Sox9) and osteogenic gene (Runx2, OCN and Col I) of ADSCs differentiated by mechanical stimulation were increased. These results indicate that osteochondral materials and dynamic environment are the key factors to improve cell regulation or signal molecule transfer function, and provide a feasible plan for osteochondral regeneration for future medical services. |
| WOS关键词 | MESENCHYMAL STEM-CELLS ; ARTICULAR-CARTILAGE ; OSTEOGENIC DIFFERENTIATION ; CHONDROGENIC DIFFERENTIATION ; EXTRACELLULAR-MATRIX ; FIBER SCAFFOLDS ; STROMAL CELLS ; SHEAR-STRESS ; GELATIN ; HYDROGEL |
| 资助项目 | National Natural Science Foundation of China[31670978/21676041/32000977] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning[20180510028] ; Fundamental Research Funds for the Central Universities[DUT20ZD206/DUT20YG118/DUT21YG113] ; Natural Science Foundation of Shandong Province[ZR2020QH188] |
| WOS研究方向 | Materials Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000814417400008 |
| 资助机构 | National Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning ; Fundamental Research Funds for the Central Universities ; Natural Science Foundation of Shandong Province |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/54205]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Li, Wenfang; Song, Kedong |
| 作者单位 | 1.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Peoples R China 2.Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China 3.Weifang Med Univ, Sch Life Sci & Technol, Weifang 261053, Peoples R China 4.Univ Sydney, ANZAC Res Inst, Burns Res Grp, Concord, NSW 2139, Australia 5.Texas A&M Univ, Artie McFerrin Dept Chem Engn, College Stn, TX 77843 USA 6.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Xueyan,Zheng, Shuangshuang,Zhang, Rui,et al. Dynamic process enhancement on chitosan/gelatin/nano-hydroxyapatite-bone derived multilayer scaffold for osteochondral tissue repair[J]. BIOMATERIALS ADVANCES,2022,133:17. |
| APA | Hu, Xueyan.,Zheng, Shuangshuang.,Zhang, Rui.,Wang, Yiwei.,Jiao, Zeren.,...&Song, Kedong.(2022).Dynamic process enhancement on chitosan/gelatin/nano-hydroxyapatite-bone derived multilayer scaffold for osteochondral tissue repair.BIOMATERIALS ADVANCES,133,17. |
| MLA | Hu, Xueyan,et al."Dynamic process enhancement on chitosan/gelatin/nano-hydroxyapatite-bone derived multilayer scaffold for osteochondral tissue repair".BIOMATERIALS ADVANCES 133(2022):17. |
入库方式： OAI收割
来源：过程工程研究所
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