Development and characterization of anti-HPV16 monoclonal antibodies for assembly of an HPV16 detection kit
文献类型：期刊论文
| 作者 | Liu, Yuying1,2,3; Zhang, Yao1; Zhang, Guifeng2; Yang, Zengmin1; Wang, Yan1; Wu, Shuming1; Chen, Dan1; Zhang, Haijiang1; Liu, Yongjiang1 |
| 刊名 | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
 |
| 出版日期 | 2022-07-27 |
| 页码 | 9 |
| 关键词 | ELISA kit HPV vaccine HPV16 monoclonal antibody |
| ISSN号 | 0885-4513 |
| DOI | 10.1002/bab.2384 |
| 英文摘要 | Quality control is very important during the development of 3-valent (16/18/58), 9-valent (6/11/16/18/31/33/45/52/58), and 15-valent human papillomavirus (HPV) vaccines (6/11/16/18/31/33/35/39/45/52/56/58/59/68). All 3-valent, 9-valent, and 15-valent HPV vaccines contain the HPV16 antigen; therefore, a detection method that can specifically identify HPV16 in vaccines is urgently required. This study aimed to develop and characterize monoclonal antibodies to assemble a highly specific HPV16 detection kit. The HPV16 L1 pentameric protein developed as an immunogen was used to prepare monoclonal antibodies. From the pool of prepared monoclonal antibodies, we selected 4G12 and 5A6 to screen and evaluate their subtypes, specificity, neutralizing activity, serum competition, binding affinity, and gene sequencing. After these characterizations, an enzyme-linked immunosorbent assay kit for these monoclonal antibodies was developed, and excellent quality was demonstrated in the assessment of linearity, repeatability, and specificity. The developed detection kit has great potential for wide use in clinical testing and quality control in vaccine production processes. |
| WOS关键词 | PAPILLOMAVIRUS ; ASSAY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000830595500001 |
| 出版者 | WILEY |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/54253]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Zhang, Haijiang; Liu, Yongjiang |
| 作者单位 | 1.Beijing Hlth Guard Biotechnol Inc, Unit 201,Block 2,Longsheng Ind Pk, Beijing 100176, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Sch Chem & Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yuying,Zhang, Yao,Zhang, Guifeng,et al. Development and characterization of anti-HPV16 monoclonal antibodies for assembly of an HPV16 detection kit[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2022:9. |
| APA | Liu, Yuying.,Zhang, Yao.,Zhang, Guifeng.,Yang, Zengmin.,Wang, Yan.,...&Liu, Yongjiang.(2022).Development and characterization of anti-HPV16 monoclonal antibodies for assembly of an HPV16 detection kit.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,9. |
| MLA | Liu, Yuying,et al."Development and characterization of anti-HPV16 monoclonal antibodies for assembly of an HPV16 detection kit".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2022):9. |
入库方式： OAI收割
来源：过程工程研究所
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