Strategies to Improve the Biosynthesis of beta-Lactam Antibiotics by Penicillin G Acylase: Progress and Prospects
文献类型：期刊论文
| 作者 | Pan, Xin1; Xu, Lei1; Li, Yaru1; Wu, Sihua2,3; Wu, Yong1; Wei, Wenping1 |
| 刊名 | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
 |
| 出版日期 | 2022-07-18 |
| 卷号 | 10页码:14 |
| 关键词 | beta-Lactam antibiotics biosynthesis penicillin G acylase enzymatic property reaction system |
| ISSN号 | 2296-4185 |
| DOI | 10.3389/fbioe.2022.936487 |
| 英文摘要 | beta-Lactam antibiotics are widely used anti-infection drugs that are traditionally synthesized via a chemical process. In recent years, with the growing demand for green alternatives, scientists have turned to enzymatic synthesis. Penicillin G acylase (PGA) is the second most commercially used enzyme worldwide with both hydrolytic and synthetic activities toward antibiotics, which has been used to manufacture the key antibiotic nucleus on an industrial level. However, the large-scale application of PGA-catalyzed antibiotics biosynthesis is still in the experimental stage because of some key limitations, such as low substrate concentration, unsatisfactory yield, and lack of superior biocatalysts. This paper systematically reviews the strategies adopted to improve the biosynthesis of beta-lactam antibiotics by adjusting the enzymatic property and manipulating the reaction system in recent 20 years, including mining of enzymes, protein engineering, solvent engineering, in situ product removal, and one-pot reaction cascade. These advances will provide important guidelines for the future use of enzymatic synthesis in the industrial production of beta-lactam antibiotics. |
| WOS关键词 | EFFICIENT ENZYMATIC-SYNTHESIS ; HIGH SUBSTRATE CONCENTRATION ; KINETICALLY CONTROLLED SYNTHESIS ; SITE-DIRECTED MUTAGENESIS ; AQUEOUS 2-PHASE SYSTEMS ; CATALYZED SYNTHESIS ; ETHYLENE-GLYCOL ; ACTIVE-SITE ; SYNTHETIC PERFORMANCE ; CEPHALEXIN SYNTHESIS |
| 资助项目 | National Natural Science Foundation of China[82000257] ; Natural Science Foundation of Jiangsu Higher Education Institutions of China[20KJB320006] ; Scientific Research Topics of Jiangsu Provincial Health Commission[M2021076] ; Social Development Project of Yangzhou City[YZ2020087] ; Social Development Project of Yangzhou City[YZ2021061] ; Young Talent Support Project of Jiangsu Provincial Association for Science and Technology |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000834569200001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Jiangsu Higher Education Institutions of China ; Scientific Research Topics of Jiangsu Provincial Health Commission ; Social Development Project of Yangzhou City ; Young Talent Support Project of Jiangsu Provincial Association for Science and Technology |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/54388]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Pan, Xin; Wu, Yong; Wei, Wenping |
| 作者单位 | 1.Yangzhou Univ, Affiliated Hosp Yangzhou Univ, Dept Cardiol, Cent Lab, Yangzhou, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 3.Gunma Univ, Grad Sch Sci & Technol, Div Mol Sci, Kiryu, Japan |
| 推荐引用方式 GB/T 7714 | Pan, Xin,Xu, Lei,Li, Yaru,et al. Strategies to Improve the Biosynthesis of beta-Lactam Antibiotics by Penicillin G Acylase: Progress and Prospects[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2022,10:14. |
| APA | Pan, Xin,Xu, Lei,Li, Yaru,Wu, Sihua,Wu, Yong,&Wei, Wenping.(2022).Strategies to Improve the Biosynthesis of beta-Lactam Antibiotics by Penicillin G Acylase: Progress and Prospects.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,10,14. |
| MLA | Pan, Xin,et al."Strategies to Improve the Biosynthesis of beta-Lactam Antibiotics by Penicillin G Acylase: Progress and Prospects".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 10(2022):14. |
入库方式： OAI收割
来源：过程工程研究所
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